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XB-ART-49971
Dev Biol. January 15, 2015; 397 (2): 175-90.

Pax8 and Pax2 are specifically required at different steps of Xenopus pronephros development.



Abstract
The respective role of Pax2 and Pax8 in early kidney development in vertebrates is poorly understood. In this report, we have studied the roles of Pax8 and Pax2 in Xenopus pronephros development using a loss-of-function approach. Our results highlight a differential requirement of these two transcription factors for proper pronephros formation. Pax8 is necessary for the earliest steps of pronephric development and its depletion leads to a complete absence of pronephric tubule. Pax2 is required after the establishment of the tubule pronephric anlage, for the expression of several terminal differentiation markers of the pronephric tubule. Neither Pax2 nor Pax8 is essential to glomus development. We further show that Pax8 controls hnf1b, but not lhx1 and Osr2, expression in the kidney field as soon as the mid-neurula stage. Pax8 is also required for cell proliferation of pronephric precursors in the kidney field. It may exert its action through the wnt/beta-catenin pathway since activation of this pathway can rescue MoPax8 induced proliferation defect and Pax8 regulates expression of the wnt pathway components, dvl1 and sfrp3. Finally, we observed that loss of pronephros in Pax8 morphants correlates with an expanded vascular/blood gene expression domain indicating that Pax8 function is important to delimit the blood/endothelial genes expression domain in the anterior part of the dorso-lateral plate.

PubMed ID: 25446030
Article link: Dev Biol.

Genes referenced: aplnr atp1b1 clcnkb cldn19 dkk1 dkk3 dvl1 dvl2 dvl3 elavl1 foxf1 frzb frzb2 hey1 hnf1b kdr lhx1 lmo2 mecom mlc1 myf5 myod1 nphs1 odc1 odf3 osr2 pax2 pax8 prkci rgn sfrp1 sfrp2 sfrp5 slc12a1 slc4a4 sox17b.1 szl tal1 wnt11b wnt4 wnt5a wnt6 wnt8a wnt9a wnt9b wt1 xk81a1

Antibodies referenced: Kidney Ab1 Kidney Ab2
Morpholinos referenced: pax2 MO2 pax2 MO3 pax8 MO1 pax8 MO2


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