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XB-ART-5039
Genes Dev July 1, 2003; 17 (13): 1663-76.

PKC delta is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements.

Kinoshita N , Iioka H , Miyakoshi A , Ueno N .


Abstract
Protein kinase C (PKC) has been implicated in the Wnt signaling pathway; however, its molecular role is poorly understood. We identified novel genes encoding delta-type PKC in the Xenopus EST databases. Loss of PKC delta function revealed that it was essential for convergent extension during gastrulation. We then examined the relationship between PKC delta and the Wnt pathway. PKC delta was translocated to the plasma membrane in response to Frizzled signaling. In addition, loss of PKC delta function inhibited the translocation of Dishevelled and the activation of c-Jun N-terminal kinase (JNK) by Frizzled. Furthermore, PKC delta formed a complex with Dishevelled, and the activation of PKC delta by phorbol ester was sufficient for Dishevelled translocation and JNK activation. Thus, PKC delta plays an essential role in the Wnt/JNK pathway by regulating the localization and activity of Dishevelled.

PubMed ID: 12842914
PMC ID: PMC196137
Article link: Genes Dev

Genes referenced: chrd.1 dvl1 dvl2 fn1 fzd7 gsc jun lgals4.2 map2k7 mapk8 myc myf5 nodal3.1 nodal3.2 otx2 prkcd sia1 tbxt wnt11 wnt8a
Antibodies: FLAG Ab2 Notochord Ab2 Somite Ab3
Morpholinos: prkcd MO1 prkcd MO2


Article Images: [+] show captions
References:
Adler, 2002, Pubmed [+]


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