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XB-ART-50416
PLoS One January 1, 2014; 9 (12): e115165.

Xenopus Nkx6.3 is a neural plate border specifier required for neural crest development.

Zhang Z , Shi Y , Zhao S , Li J , Li C , Mao B .


Abstract
In vertebrates, the neural plate border (NPB) is established by a group of transcription factors including Dlx3, Msx1 and Zic1. The crosstalk between these NPB specifiers governs the separation of the NPB region into placode and neural crest (NC) territories and also their further differentiation. Understanding the mechanisms of NPB formation and NC development is critical for our knowledge of related human diseases. Here we identified Nkx6.3, a transcription factor of the Nkx family, as a new NPB specifier required for neural crest development in Xenopus embryos. XNkx6.3 is expressed in the ectoderm of the neural plate border region at neurula stages, covering the epidermis, placode and neural crest territories, but not the neural plate. Inhibition of Nkx6.3 by dominant negative construct or specific morpholino leads to neural crest defects, while overexpression of Nkx6.3 induces ectopic neural crest in the anterior neural fold. In animal caps, Nkx6.3 alone is able to initiate the whole neural crest regulatory network and induces neural crest fate robustly. We showed that overexpression of Nkx6.3 affects multiple signaling pathways, creating a high-Wnt, low-BMP environment required for neural crest development. Gain- and loss-of-function of Nkx6.3 have compound effects on the expression of known NPB genes, which is largely opposite to that of Dlx3. Overexpression of Dlx3 blocks the NC inducing activity of Nkx6.3. The crosstalk between Nkx6.3, Dlx3 and Msx1 is likely crucial for proper NPB formation and neural crest development in Xenopus.

PubMed ID: 25531524
PMC ID: PMC4274032
Article link: PLoS One

Genes referenced: dlx3 dlx5 eya1 fgf8 foxd3 fzd3 hdc lgals4.2 msx1 nkx6-3 npb pax3 six1 snai2 sox2 wnt8a zic1
Morpholinos: nkx6-3 MO1


Article Images: [+] show captions
References:
Ahrens, 2005, Pubmed, Xenbase [+]


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