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Cell Cycle
2014 Jan 01;1311:1727-36. doi: 10.4161/cc.28630.
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Phosphorylation-mediated stabilization of Bora in mitosis coordinates Plx1/Plk1 and Cdk1 oscillations.
Feine O
,
Hukasova E
,
Bruinsma W
,
Freire R
,
Fainsod A
,
Gannon J
,
Mahbubani HM
,
Lindqvist A
,
Brandeis M
.
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Cdk1 and Plk1/Plx1 activation leads to their inactivation through negative feedback loops. Cdk1 deactivates itself by activating the APC/C, consequently generating embryonic cell cycle oscillations. APC/C inhibition by the mitotic checkpoint in somatic cells and the cytostatic factor (CSF) in oocytes sustain the mitotic state. Plk1/Plx1 targets its co-activator Bora for degradation, but it remains unclear how embryonic oscillations in Plx1 activity are generated, and how Plk1/Plx1 activity is sustained during mitosis. We show that Plx1-mediated degradation of Bora in interphase generates oscillations in Plx1 activity and is essential for development. In CSF extracts, phosphorylation of Bora on the Cdk consensus site T52 blocks Bora degradation. Upon fertilization, Calcineurin dephosphorylates T52, triggering Plx1 oscillations. Similarly, we find that GFP-Bora is degraded when Plk1 activity spreads to somatic cell cytoplasm before mitosis. Interestingly, GFP-Bora degradation stops upon mitotic entry when Cdk1 activity is high. We hypothesize that Cdk1 controls Bora through an incoherent feedforward loop synchronizing the activities of mitotic kinases.
Akopyan,
Assessing kinetics from fixed cells reveals activation of the mitotic entry network at the S/G2 transition.
2014, Pubmed
Akopyan,
Assessing kinetics from fixed cells reveals activation of the mitotic entry network at the S/G2 transition.
2014,
Pubmed
Bruinsma,
Bora and Aurora-A continue to activate Plk1 in mitosis.
2014,
Pubmed
Chan,
Plk1 regulates mitotic Aurora A function through betaTrCP-dependent degradation of hBora.
2008,
Pubmed
Coster,
The DNA damage response mediator MDC1 directly interacts with the anaphase-promoting complex/cyclosome.
2007,
Pubmed
Descombes,
The polo-like kinase Plx1 is required for M phase exit and destruction of mitotic regulators in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
Esmaili,
Regulation of the ATM-activator protein Aven by CRM1-dependent nuclear export.
2010,
Pubmed
,
Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Golsteyn,
Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function.
1995,
Pubmed
Hinchcliffe,
Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase
Holt,
Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.
2009,
Pubmed
Hukasova,
Monitoring kinase and phosphatase activities through the cell cycle by ratiometric FRET.
2012,
Pubmed
Hutterer,
Mitotic activation of the kinase Aurora-A requires its binding partner Bora.
2006,
Pubmed
Kelm,
Cell cycle-regulated phosphorylation of the Xenopus polo-like kinase Plx1.
2002,
Pubmed
,
Xenbase
Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Lee,
Glycogen synthase kinase 3 β activity is required for hBora/Aurora A-mediated mitotic entry.
2013,
Pubmed
Lindqvist,
The decision to enter mitosis: feedback and redundancy in the mitotic entry network.
2009,
Pubmed
Littlepage,
Identification of a new APC/C recognition domain, the A box, which is required for the Cdh1-dependent destruction of the kinase Aurora-A during mitotic exit.
2002,
Pubmed
,
Xenbase
Macůrek,
Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery.
2008,
Pubmed
Mangan,
Structure and function of the feed-forward loop network motif.
2003,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Medema,
Boosting and suppressing mitotic phosphorylation.
2011,
Pubmed
Mochida,
Calcineurin is required to release Xenopus egg extracts from meiotic M phase.
2007,
Pubmed
,
Xenbase
Mochida,
Aurora borealis wraps Plk1 and CDK together.
2014,
Pubmed
Moshe,
Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome.
2004,
Pubmed
,
Xenbase
Murray,
Dominoes and clocks: the union of two views of the cell cycle.
1989,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Novák,
Design principles of biochemical oscillators.
2008,
Pubmed
Nurse,
Genetic control of cell size at cell division in yeast.
1975,
Pubmed
Qian,
Mitotic effects of a constitutively active mutant of the Xenopus polo-like kinase Plx1.
1999,
Pubmed
,
Xenbase
Qian,
The polo-like kinase Plx1 is required for activation of the phosphatase Cdc25C and cyclin B-Cdc2 in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Seki,
Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progression.
2008,
Pubmed
Seki,
Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry.
2008,
Pubmed
,
Xenbase
Wu,
PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylation.
2009,
Pubmed
,
Xenbase
Yang,
The Cdk1-APC/C cell cycle oscillator circuit functions as a time-delayed, ultrasensitive switch.
2013,
Pubmed
,
Xenbase
Zhai,
Bora regulates meiotic spindle assembly and cell cycle during mouse oocyte meiosis.
2013,
Pubmed
