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XB-ART-50554
J Nanobiotechnology. April 29, 2015; 13 31.

Quantum dot assisted tracking of the intracellular protein Cyclin E in Xenopus laevis embryos.

Brandt YI , Mitchell T , Smolyakov GA , Osiński M , Hartley RS .


Abstract
Luminescent semiconductor nanocrystals, also known as quantum dots (QD), possess highly desirable optical properties that account for development of a variety of exciting biomedical techniques. These properties include long-term stability, brightness, narrow emission spectra, size tunable properties and resistance to photobleaching. QD have many promising applications in biology and the list is constantly growing. These applications include DNA or protein tagging for in vitro assays, deep-tissue imaging, fluorescence resonance energy transfer (FRET), and studying dynamics of cell surface receptors, among others. Here we explored the potential of QD-mediated labeling for the purpose of tracking an intracellular protein inside live cells. We manufactured dihydrolipoic acid (DHLA)-capped CdSe-ZnS core-shell QD, not available commercially, and coupled them to the cell cycle regulatory protein Cyclin E. We then utilized the QD fluorescence capabilities for visualization of Cyclin E trafficking within cells of Xenopus laevis embryos in real time. These studies provide "proof-of-concept" for this approach by tracking QD-tagged Cyclin E within cells of developing embryos, before and during an important developmental period, the midblastula transition. Importantly, we show that the attachment of QD to Cyclin E did not disrupt its proper intracellular distribution prior to and during the midblastula transition. The fate of the QD after cyclin E degradation following the midblastula transition remains unknown.

PubMed ID: 25925383
PMC ID: PMC4424550
Article link: J Nanobiotechnology.
Grant support: R01 CA095898 NCI NIH HHS , S10 RR016918 NCRR NIH HHS , P30 CA118100 NCI NIH HHS , 1 S10 RR14668 NCRR NIH HHS , S10 RR19287 NCRR NIH HHS , P20 RR11830 NCRR NIH HHS , S10 RR019287 NCRR NIH HHS , R01CA095898 NCI NIH HHS



References:
Andrews, 2008, Pubmed[+]


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