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Cell Mol Life Sci
2015 Sep 01;7218:3575-86. doi: 10.1007/s00018-015-1905-3.
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Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs.
Shi Z
,
Zhao C
,
Yang Y
,
Teng H
,
Guo Y
,
Ma M
,
Guo X
,
Zhou Z
,
Huo R
,
Zhou Q
.
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In mammals, pronucleus formation, a landmark event for egg activation and fertilization, is critical for embryonic development. However, the mechanisms underlying pronucleus formation remain unclear. Increasing evidence has shown that the transition from a mature egg to a developing embryo and the early steps of development are driven by the control of maternal cytoplasmic factors. Herein, a two-dimensional-electrophoresis-based proteomic approach was used in metaphase II and parthenogenetically activated mouse eggs to search for maternal proteins involved in egg activation, one of which was poly(rC)-binding protein 1 (PCBP1). Phosphoprotein staining indicated that PCBP1 displayed dephosphorylation in parthenogenetically activated egg, which possibly boosts its ability to bind to mRNAs. We identified 75 mRNAs expressed in mouse eggs that contained the characteristic PCBP1-binding CU-rich sequence in the 3'-UTR. Among them, we focused on H2a.x mRNA, as it was closely related to pronucleus formation in Xenopus oocytes. Further studies suggested that PCBP1 could bind to H2a.x mRNA and enhance its stability, thus promoting mouse pronucleus formation during parthenogenetic activation of murine eggs, while the inhibition of PCBP1 evidently retarded pronucleus formation. In summary, these data propose that PCBP1 may serve as a novel maternal factor that is required for determining the normal timing of pronucleus formation.
Agrawal,
Development of a simplified, economical polyacrylamide gel staining protocol for phosphoproteins.
2005, Pubmed
Agrawal,
Development of a simplified, economical polyacrylamide gel staining protocol for phosphoproteins.
2005,
Pubmed
Ahnesjö,
The IMRT information process-mastering the degrees of freedom in external beam therapy.
2006,
Pubmed
Beavis,
Is tomotherapy the future of IMRT?
2004,
Pubmed
Bohsung,
IMRT treatment planning:- a comparative inter-system and inter-centre planning exercise of the ESTRO QUASIMODO group.
2005,
Pubmed
Bratengeier,
Applications of two-step intensity modulated arc therapy.
2001,
Pubmed
Bratengeier,
A comparison between 2-Step IMRT and conventional IMRT planning.
2007,
Pubmed
Cao,
Comparison of plan quality provided by intensity-modulated arc therapy and helical tomotherapy.
2007,
Pubmed
Carol,
Initial clinical experience with the Peacock intensity modulation of a 3-D conformal radiation therapy system.
1997,
Pubmed
Clift,
Restarting life: fertilization and the transition from meiosis to mitosis.
2013,
Pubmed
Collas,
Cytoplasmic control of nuclear assembly.
1998,
Pubmed
Czyzyk-Krzeska,
Identification of the poly(C) binding protein in the complex associated with the 3' untranslated region of erythropoietin messenger RNA.
1999,
Pubmed
Dai,
Selective requirement for Cdc25C protein synthesis during meiotic progression in porcine oocytes.
2000,
Pubmed
Dimitrov,
Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
1994,
Pubmed
,
Xenbase
Duthoy,
Clinical implementation of intensity-modulated arc therapy (IMAT) for rectal cancer.
2004,
Pubmed
Earl,
Inverse planning for intensity-modulated arc therapy using direct aperture optimization.
2003,
Pubmed
Edlund,
IMRT for the treatment of prostate cancer: a comparison of a forward-planned technique and an inverse-planned technique utilizing a dose gradient method.
2004,
Pubmed
Evans,
Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo.
2003,
Pubmed
Evsikov,
Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
2006,
Pubmed
,
Xenbase
Fenwick,
Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro.
2002,
Pubmed
Fiorino,
Simultaneous integrated boost (SIB) for nasopharynx cancer with helical tomotherapy. A planning study.
2007,
Pubmed
Fiorino,
Significant improvement in normal tissue sparing and target coverage for head and neck cancer by means of helical tomotherapy.
2006,
Pubmed
Holcik,
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
1997,
Pubmed
Horner,
Transitioning from egg to embryo: triggers and mechanisms of egg activation.
2008,
Pubmed
Huang,
Construction of a proteome profile and functional analysis of the proteins involved in the initiation of mouse spermatogenesis.
2008,
Pubmed
Inoue,
Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes.
2014,
Pubmed
Kim,
Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.
2000,
Pubmed
Kleinschmidt,
DNA-dependent phosphorylation of histone H2A.X during nucleosome assembly in Xenopus laevis oocytes: involvement of protein phosphorylation in nucleosome spacing.
1991,
Pubmed
,
Xenbase
Ko,
Poly C binding protein, a single-stranded DNA binding protein, regulates mouse mu-opioid receptor gene expression.
2005,
Pubmed
Koyama,
Aging-related changes in in vitro-matured bovine oocytes: oxidative stress, mitochondrial activity and ATP content after nuclear maturation.
2014,
Pubmed
Krauchunas,
Molecular changes during egg activation.
2013,
Pubmed
Leffers,
Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.
1995,
Pubmed
Li,
Maternal control of early mouse development.
2010,
Pubmed
Lim,
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.
2006,
Pubmed
Lonergan,
Effect of time interval from insemination to first cleavage on the developmental characteristics, sex ratio and pregnancy rate after transfer of bovine embryos.
1999,
Pubmed
Ma,
Optimized intensity-modulated arc therapy for prostate cancer treatment.
2001,
Pubmed
Ma,
Protein expression profile of the mouse metaphase-II oocyte.
2008,
Pubmed
Mackie,
Tomotherapy: a new concept for the delivery of dynamic conformal radiotherapy.
1994,
Pubmed
Malcuit,
Calcium oscillations and mammalian egg activation.
2006,
Pubmed
McLaren,
Genetic effects on the timing of early development in the mouse.
1973,
Pubmed
Meng,
Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1.
2007,
Pubmed
Mignone,
UTRdb and UTRsite: a collection of sequences and regulatory motifs of the untranslated regions of eukaryotic mRNAs.
2005,
Pubmed
Mihai,
Inverse vs. forward breast IMRT planning.
2005,
Pubmed
Nakamura,
XCS-1, a maternally expressed gene product involved in regulating mitosis in Xenopus.
2000,
Pubmed
,
Xenbase
Otto,
Volumetric modulated arc therapy: IMRT in a single gantry arc.
2008,
Pubmed
Paulding,
Regulation of tyrosine hydroxylase mRNA stability by protein-binding, pyrimidine-rich sequence in the 3'-untranslated region.
1999,
Pubmed
Perreault,
The timing of hamster sperm nuclear decondensation and male pronucleus formation is related to sperm nuclear disulfide bond content.
1987,
Pubmed
Pickering,
Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo.
2003,
Pubmed
Pickering,
Bag-1 internal ribosome entry segment activity is promoted by structural changes mediated by poly(rC) binding protein 1 and recruitment of polypyrimidine tract binding protein 1.
2004,
Pubmed
Poccia,
Nuclear envelope dynamics during male pronuclear development.
1997,
Pubmed
Racki,
CPEB controls oocyte growth and follicle development in the mouse.
2006,
Pubmed
Redon,
Histone H2A variants H2AX and H2AZ.
2002,
Pubmed
Rochet,
Helical tomotherapy as a new treatment technique for whole abdominal irradiation.
2008,
Pubmed
Shepard,
An arc-sequencing algorithm for intensity modulated arc therapy.
2007,
Pubmed
Shepard,
Direct aperture optimization: a turnkey solution for step-and-shoot IMRT.
2002,
Pubmed
Sterzing,
Helical tomotherapy. Experiences of the first 150 patients in Heidelberg.
2008,
Pubmed
Stitzel,
Regulation of the oocyte-to-zygote transition.
2007,
Pubmed
Thiele,
RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis.
2004,
Pubmed
Tuteja,
Molecular mechanisms of DNA damage and repair: progress in plants.
2001,
Pubmed
Wang,
Proteome of mouse oocytes at different developmental stages.
2010,
Pubmed
Weiss,
Erythroid cell-specific determinants of alpha-globin mRNA stability.
1994,
Pubmed
Weiss,
Erythroid cell-specific mRNA stability elements in the alpha 2-globin 3' nontranslated region.
1995,
Pubmed
Welsh,
Helical tomotherapy: an innovative technology and approach to radiation therapy.
2003,
Pubmed
Wiezorek,
Experimental determination of peripheral doses for different IMRT techniques delivered by a Siemens linear accelerator.
2008,
Pubmed
Wong,
Intensity-modulated arc therapy simplified.
2002,
Pubmed
Wu,
Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development.
2014,
Pubmed
Xia,
PCBP1 is required for maintenance of the transcriptionally silent state in fully grown mouse oocytes.
2012,
Pubmed
Yeap,
Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.
2002,
Pubmed
Yu,
Intensity-modulated arc therapy with dynamic multileaf collimation: an alternative to tomotherapy.
1996,
Pubmed
Zhong,
Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C.
2005,
Pubmed
Zuccotti,
Chromatin organisation and nuclear architecture in growing mouse oocytes.
2005,
Pubmed
