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XB-ART-50752
Dev Dyn August 1, 2015; 244 (8): 988-1013.

Functional analysis of Hairy genes in Xenopus neural crest initial specification and cell migration.

Vega-López GA , Bonano M , Tríbulo C , Fernández JP , Agüero TH , Aybar MJ .


Abstract
Neural crest formation is one of the fundamental processes in the early stages of embryonic development in vertebrates. This transient and multipotent embryonic cell population is able to generate a variety of tissues and cell types in the adult body. hairy genes are transcription factors that contain a basic helix-loop-helix domain which binds to DNA. In Xenopus three hairy genes are known: hairy1, hairy2a, and hairy2b. The requirement of hairy genes was explored in early neural crest development although the late requirements of these genes during neural crest maintenance, migration and derivatives formation are still unknown. In this work, we extended the analysis of Xenopus hairy genes expression patterns and described new domains of expression. Functional analysis showed that hairy genes are required for the induction and migration of the neural crest and for the control of apoptosis. Moreover, we showed that hairy genes function as transcriptional repressors and that they are down-regulated by bone morphogenetic protein-Smad signaling and positively regulated by the Notch/Delta-Su(h) pathway. Our results indicate that hairy genes have a functional equivalence between them and that they are required for multiple processes during neural crest development.

PubMed ID: 25997789
Article link: Dev Dyn

Genes referenced: bmp4 bmpr1a dll1 eef1a2 foxd3 hes1 hes4 krt12.4 notch1 pax3 smad4.1 smad4.2 snai1 snai2 sox2
Morpholinos: hes4 MO5


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