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XB-ART-50935
Biochem Cell Biol August 1, 2015; 93 (4): 396-404.

Developmental role of plk4 in Xenopus laevis and Danio rerio: implications for Seckel Syndrome.

Rapchak CE , Patel N , Hudson J , Crawford M .


Abstract
The polo-like kinases are a family of conserved serine/threonine kinases that play multiple roles in regulation of the cell cycle. Unlike its four other family members, the role of Plk4 in embryonic development has not been well characterized. In mice, Plk4(-)(/)(-) embryos arrest at E7.5, just prior to the initiation of somitogenesis. This has led to the hypothesis that Plk4 expression may be essential to somitogenesis. Recently characterized human mutations lead to Seckel Syndrome. Riboprobe in situ hybridization revealed that plk4 is ubiquitously expressed during early stages of development of Xenopus and Danio; in later stages, expression in frogs restricts to somites as well as eye, otic vesicle, and branchial arch, and brain. Expression patterns in fish remain ubiquitous. Both somite and eye development require planar cell polarity, and disruption of plk4 function in frog by means of morpholino-mediated translational knockdown yields orientational disorganization of both these structures. These results provide the first steps in defining a new role for plk4 in organogenesis and implies a role in planar cell polarity, segmentation, and in recently described PLK4 mutations in human.

PubMed ID: 26150138
Article link: Biochem Cell Biol

Genes referenced: hbg1 hes4 notch1 pax6 pitx3 plk4 rax tubg1
Antibodies: Itgb1 Ab1 Somite Ab1 Tubg1 Ab4
Morpholinos: hbg1 cMO1 plk4 MO1 plk4 MO2

Disease Ontology terms: Seckel syndrome
OMIMs: SECKEL SYNDROME 1; SCKL1

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