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XB-ART-50977
Dev Biol September 15, 2015; 405 (2): 214-24.

A novel role for Celf1 in vegetal RNA localization during Xenopus oogenesis.

Bauermeister D , Claußen M , Pieler T .


Abstract
The localization of certain mRNAs to the vegetal cortex of Xenopus oocytes is of crucial importance for germ cell development and early embryonic patterning. Vegetal RNA localization is mediated by cis-acting RNA localization elements (LE). Several proteins assemble on the RNA LE and direct transport to the vegetal cortex. Although a number of localization RNP components have been identified, their full composition is unknown. In an RNA affinity purification approach, using the dead end 1 (dnd1) RNA LE, we identified Xenopus Celf1 as a novel component of vegetal localization RNP complexes. Celf1 is part of an RNP complex together with known vegetal localization factors and shows specific interactions with LEs from several but not all vegetally localizing RNAs. Immunostaining experiments reveal co-localization of Celf1 with vegetally localizing RNA and with known localization factors. Inhibition of Celf1 protein binding by localization element mutagenesis as well as Celf1 overexpression interfere with vegetal RNA localization. These results argue for a role of Celf1 in vegetal RNA localization during Xenopus oogenesis.

PubMed ID: 26164657
Article link: Dev Biol

Genes referenced: celf1 dnd1 elavl1 elavl2 gapdh gdf1 grip2 hbg1 hnrnpab igf2bp3 ins lmnb1 myc ptbp1 spire1 stau1 tbx2 trim36 vegt velo1
Antibodies: Celf1 Ab1 Elavl Ab 2 FLAG Ab2 Igf2bp3 Ab1 Myc Ab12 Stau1 Ab2


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