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Curr Biol. August 17, 2015; 25 (16): 2177-83.

Zeta-Tubulin Is a Member of a Conserved Tubulin Module and Is a Component of the Centriolar Basal Foot in Multiciliated Cells.

There are six members of the tubulin superfamily in eukaryotes. Alpha- and beta-tubulin form a heterodimer that polymerizes to form microtubules, and gamma-tubulin nucleates microtubules as a component of the gamma-tubulin ring complex. Alpha-, beta-, and gamma-tubulin are conserved in all eukaryotes. In contrast, delta- and epsilon-tubulin are conserved in many, but not all, eukaryotes and are associated with centrioles, although their molecular function is unclear. Zeta-tubulin is the sixth and final member of the tubulin superfamily and is largely uncharacterized. We find that zeta-, epsilon-, and delta-tubulin form an evolutionarily co-conserved module, the ZED module, that has been lost at several junctions in eukaryotic evolution and that zeta- and delta-tubulin are evolutionarily interchangeable. Humans lack zeta-tubulin but have delta-tubulin. In Xenopus multiciliated cells, zeta-tubulin is a component of the basal foot, a centriolar appendage that connects centrioles to the apical cytoskeleton, and co-localizes there with epsilon-tubulin. Depletion of zeta-tubulin results in disorganization of centriole distribution and polarity in multiciliated cells. In contrast with multiciliated cells, zeta-tubulin in cycling cells does not localize to centrioles and is associated with the TRiC/CCT cytoplasmic chaperone complex. We conclude that zeta-tubulin facilitates interactions between the centrioles and the apical cytoskeleton as a component of the basal foot in differentiated cells and propose that the ZED tubulins are important for centriole functionalization and orientation of centrioles with respect to cellular polarity axes.

PubMed ID: 26234217
PMC ID: PMC4546511
Article link: Curr Biol.
Grant support: R01 GM052022 NIGMS NIH HHS , F32 GM103146 NIGMS NIH HHS , T32 CA009302 NCI NIH HHS , T32 CA09302 NCI NIH HHS , 1S10RR026780-01 NCRR NIH HHS , R01HL117164 NHLBI NIH HHS , R01GM052022 NIGMS NIH HHS , R01 HL117164 NHLBI NIH HHS , F32GM103146 NIGMS NIH HHS , S10 RR026780 NCRR NIH HHS

Genes referenced: actb cct2 cetn3 gapdh gnl3 marveld2 pcyt1a picalm.1 tuba4b tubd1 tube1 tubg1 tubz1

Antibodies referenced: Cetn3 Ab1 H3f3a Ab14 Tuba4b Ab2 Tuba4b Ab5 Tubg1 Ab4 Tubz1 Ab1
Morpholinos referenced: Tubz1 MO1 Tubz1 MO2 Tubz1 MO3

Article Images: [+] show captions

Aubusson-Fleury, 2013, Pubmed[+]

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