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Development
2015 Oct 01;14219:3429-39. doi: 10.1242/dev.121384.
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Control of vertebrate core planar cell polarity protein localization and dynamics by Prickle 2.
Butler MT
,
Wallingford JB
.
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Planar cell polarity (PCP) is a ubiquitous property of animal tissues and is essential for morphogenesis and homeostasis. In most cases, this fundamental property is governed by a deeply conserved set of 'core PCP' proteins, which includes the transmembrane proteins Van Gogh-like (Vangl) and Frizzled (Fzd), as well as the cytoplasmic effectors Prickle (Pk) and Dishevelled (Dvl). Asymmetric localization of these proteins is thought to be central to their function, and understanding the dynamics of these proteins is an important challenge in developmental biology. Among the processes that are organized by the core PCP proteins is the directional beating of cilia, such as those in the vertebrate node, airway and brain. Here, we exploit the live imaging capabilities of Xenopus to chart the progressive asymmetric localization of fluorescent reporters of Dvl1, Pk2 and Vangl1 in a planar polarized ciliated epithelium. Using this system, we also characterize the influence of Pk2 on the asymmetric dynamics of Vangl1 at the cell cortex, and we define regions of Pk2 that control its own localization and those impacting Vangl1. Finally, our data reveal a striking uncoupling of Vangl1 and Dvl1 asymmetry. This study advances our understanding of conserved PCP protein functions and also establishes a rapid, tractable platform to facilitate future in vivo studies of vertebrate PCP protein dynamics.
Adler,
The domineering non-autonomy of frizzled and van Gogh clones in the Drosophila wing is a consequence of a disruption in local signaling.
2000, Pubmed
Adler,
The domineering non-autonomy of frizzled and van Gogh clones in the Drosophila wing is a consequence of a disruption in local signaling.
2000,
Pubmed
Adler,
The genetic control of tissue polarity in Drosophila.
1992,
Pubmed
Adler,
Tissue polarity points from cells that have higher Frizzled levels towards cells that have lower Frizzled levels.
1997,
Pubmed
Aigouy,
Cell flow reorients the axis of planar polarity in the wing epithelium of Drosophila.
2010,
Pubmed
Amonlirdviman,
Mathematical modeling of planar cell polarity to understand domineering nonautonomy.
2005,
Pubmed
Axelrod,
Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling.
2001,
Pubmed
Bastock,
Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
2003,
Pubmed
Billett,
Fine structural changes in the differentiating epidermis of Xenopus laevis embryos.
1971,
Pubmed
,
Xenbase
Boutin,
A dual role for planar cell polarity genes in ciliated cells.
2014,
Pubmed
Brooks,
Multiciliated cells.
2014,
Pubmed
Chen,
Asymmetric homotypic interactions of the atypical cadherin flamingo mediate intercellular polarity signaling.
2008,
Pubmed
Cho,
Clustering and negative feedback by endocytosis in planar cell polarity signaling is modulated by ubiquitinylation of prickle.
2015,
Pubmed
Chung,
Coordinated genomic control of ciliogenesis and cell movement by RFX2.
2014,
Pubmed
,
Xenbase
Collier,
The WD40 repeat protein fritz links cytoskeletal planar polarity to frizzled subcellular localization in the Drosophila epidermis.
2005,
Pubmed
Cui,
Wdpcp, a PCP protein required for ciliogenesis, regulates directional cell migration and cell polarity by direct modulation of the actin cytoskeleton.
2013,
Pubmed
Das,
Diego interacts with Prickle and Strabismus/Van Gogh to localize planar cell polarity complexes.
2004,
Pubmed
Daulat,
Mink1 regulates β-catenin-independent Wnt signaling via Prickle phosphorylation.
2012,
Pubmed
,
Xenbase
Deans,
Asymmetric distribution of prickle-like 2 reveals an early underlying polarization of vestibular sensory epithelia in the inner ear.
2007,
Pubmed
Dubaissi,
A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis.
2014,
Pubmed
,
Xenbase
Dubaissi,
Embryonic frog epidermis: a model for the study of cell-cell interactions in the development of mucociliary disease.
2011,
Pubmed
,
Xenbase
Goodrich,
Principles of planar polarity in animal development.
2011,
Pubmed
Gray,
Planar cell polarity: coordinating morphogenetic cell behaviors with embryonic polarity.
2011,
Pubmed
Gray,
The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development.
2009,
Pubmed
,
Xenbase
Gubb,
Genes controlling cellular polarity in Drosophila.
1993,
Pubmed
Gubb,
A genetic analysis of the determination of cuticular polarity during development in Drosophila melanogaster.
1982,
Pubmed
Guirao,
Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia.
2010,
Pubmed
Guo,
A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network.
2013,
Pubmed
Jakobsen,
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
2011,
Pubmed
Jenny,
Prickle and Strabismus form a functional complex to generate a correct axis during planar cell polarity signaling.
2003,
Pubmed
,
Xenbase
Jenny,
Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding.
2005,
Pubmed
Kim,
Planar cell polarity acts through septins to control collective cell movement and ciliogenesis.
2010,
Pubmed
,
Xenbase
König,
Planar polarity in the ciliated epidermis of Xenopus embryos.
1993,
Pubmed
,
Xenbase
Lee,
The function of the frizzled pathway in the Drosophila wing is dependent on inturned and fuzzy.
2002,
Pubmed
Lin,
Molecular dissection of Drosophila Prickle isoforms distinguishes their essential and overlapping roles in planar cell polarity.
2009,
Pubmed
Liu,
Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a.
2014,
Pubmed
Merte,
Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure.
2010,
Pubmed
Mitchell,
The PCP pathway instructs the planar orientation of ciliated cells in the Xenopus larval skin.
2009,
Pubmed
,
Xenbase
Mitchell,
A positive feedback mechanism governs the polarity and motion of motile cilia.
2007,
Pubmed
,
Xenbase
Narimatsu,
Regulation of planar cell polarity by Smurf ubiquitin ligases.
2009,
Pubmed
Okumura,
3p interstitial deletion including PRICKLE2 in identical twins with autistic features.
2014,
Pubmed
Park,
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.
2008,
Pubmed
,
Xenbase
Park,
Ciliogenesis defects in embryos lacking inturned or fuzzy function are associated with failure of planar cell polarity and Hedgehog signaling.
2006,
Pubmed
,
Xenbase
Quigley,
Specification of ion transport cells in the Xenopus larval skin.
2011,
Pubmed
,
Xenbase
Seifert,
Frizzled/PCP signalling: a conserved mechanism regulating cell polarity and directed motility.
2007,
Pubmed
Shindo,
PCP and septins compartmentalize cortical actomyosin to direct collective cell movement.
2014,
Pubmed
,
Xenbase
Sokol,
Analysis of Dishevelled signalling pathways during Xenopus development.
1996,
Pubmed
,
Xenbase
Song,
Planar cell polarity breaks bilateral symmetry by controlling ciliary positioning.
2010,
Pubmed
Sowers,
Disruption of the non-canonical Wnt gene PRICKLE2 leads to autism-like behaviors with evidence for hippocampal synaptic dysfunction.
2013,
Pubmed
Struhl,
Dissecting the molecular bridges that mediate the function of Frizzled in planar cell polarity.
2012,
Pubmed
Strutt,
The planar polarity pathway.
2008,
Pubmed
Strutt,
Nonautonomous planar polarity patterning in Drosophila: dishevelled-independent functions of frizzled.
2002,
Pubmed
Strutt,
Asymmetric localization of frizzled and the establishment of cell polarity in the Drosophila wing.
2001,
Pubmed
Strutt,
Strabismus promotes recruitment and degradation of farnesylated prickle in Drosophila melanogaster planar polarity specification.
2013,
Pubmed
Strutt,
Differential activities of the core planar polarity proteins during Drosophila wing patterning.
2007,
Pubmed
Strutt,
Dynamics of core planar polarity protein turnover and stable assembly into discrete membrane subdomains.
2011,
Pubmed
Strutt,
A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
2013,
Pubmed
Strutt,
Differential stability of flamingo protein complexes underlies the establishment of planar polarity.
2008,
Pubmed
Strutt,
Asymmetric localisation of planar polarity proteins: Mechanisms and consequences.
2009,
Pubmed
Sweede,
Structural and membrane binding properties of the prickle PET domain.
2008,
Pubmed
Takeuchi,
The prickle-related gene in vertebrates is essential for gastrulation cell movements.
2003,
Pubmed
,
Xenbase
Tao,
Mutations in prickle orthologs cause seizures in flies, mice, and humans.
2011,
Pubmed
Torban,
Genetic interaction between members of the Vangl family causes neural tube defects in mice.
2008,
Pubmed
Tree,
Prickle mediates feedback amplification to generate asymmetric planar cell polarity signaling.
2002,
Pubmed
Vinson,
Directional non-cell autonomy and the transmission of polarity information by the frizzled gene of Drosophila.
,
Pubmed
Vladar,
Microtubules enable the planar cell polarity of airway cilia.
2012,
Pubmed
Walentek,
A novel serotonin-secreting cell type regulates ciliary motility in the mucociliary epidermis of Xenopus tadpoles.
2014,
Pubmed
,
Xenbase
Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase
Wallingford,
Planar cell polarity and the developmental control of cell behavior in vertebrate embryos.
2012,
Pubmed
Wallingford,
Planar cell polarity signaling, cilia and polarized ciliary beating.
2010,
Pubmed
Wang,
The proteins encoded by the Drosophila Planar Polarity Effector genes inturned, fuzzy and fritz interact physically and can re-pattern the accumulation of "upstream" Planar Cell Polarity proteins.
2014,
Pubmed
Wen,
Planar cell polarity pathway genes and risk for spina bifida.
2010,
Pubmed
,
Xenbase
Werner,
Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells.
2011,
Pubmed
,
Xenbase
Wong,
Tissue polarity genes of Drosophila regulate the subcellular location for prehair initiation in pupal wing cells.
1993,
Pubmed
Wu,
The frizzled extracellular domain is a ligand for Van Gogh/Stbm during nonautonomous planar cell polarity signaling.
2008,
Pubmed
Yin,
Comparison of phenotypes between different vangl2 mutants demonstrates dominant effects of the Looptail mutation during hair cell development.
2012,
Pubmed
Zeng,
PCP effector gene Inturned is an important regulator of cilia formation and embryonic development in mammals.
2010,
Pubmed
