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XB-ART-51179
PLoS One January 1, 2015; 10 (8): e0136408.

A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis.

Carmel MS , Kahane N , Oberman F , Miloslavski R , Sela-Donenfeld D , Kalcheim C , Yisraeli JK .


Abstract
VICKZ (IGF2BP1,2,3/ZBP1/Vg1RBP/IMP1,2,3) proteins bind RNA and help regulate many RNA-mediated processes. In the midbrain region of early chick embryos, VICKZ is expressed in the neural folds and along the basal surface of the neural epithelium, but, upon neural tube closure, is down-regulated in prospective cranial neural crest (CNC) cells, concomitant with their emigration and epithelial-to-mesenchymal transition (EMT). Electroporation of constructs that modulate cVICKZ expression demonstrates that this down-regulation is both necessary and sufficient for CNC EMT. These results suggest that VICKZ down-regulation in CNC cell-autonomously promotes EMT and migration. Reduction of VICKZ throughout the embryo, however, inhibits CNC migration non-cell-autonomously, as judged by transplantation experiments in Xenopus embryos. Given the positive role reported for VICKZ proteins in promoting cell migration of chick embryo fibroblasts and many types of cancer cells, we have begun to look for specific mRNAs that could mediate context-specific differences. We report here that the laminin receptor, integrin alpha 6, is down-regulated in the dorsal neural tube when CNC cells emigrate, this process is mediated by cVICKZ, and integrin alpha 6 mRNA is found in VICKZ ribonucleoprotein complexes. Significantly, prolonged inhibition of cVICKZ in either the neural tube or the nascent dermomyotome sheet, which also dynamically expresses cVICKZ, induces disruption of these epithelia. These data point to a previously unreported role for VICKZ in maintaining epithelial integrity.

PubMed ID: 26317350
PMC ID: PMC4552865
Article link: PLoS One

Genes referenced: b3gat1l igf2bp3 imp3 itga6 itk pax7 rpsa zbp1
Morpholinos: igf2bp3 MO1


Article Images: [+] show captions
References:
Adereth, 2005, Pubmed [+]


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