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J Cell Biol June 22, 2015; 209 (6): 789-801.

A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance.

Westhorpe FG , Fuller CJ , Straight AF .

Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric chromatin that direct new CENP-A assembly remain unclear. Here, we developed a cell-free CENP-A assembly system that enabled the study of chromatin-bound CENP-A and soluble CENP-A separately. We show that two distinct domains of CENP-A within existing CENP-A nucleosomes are required for new CENP-A assembly and that CENP-A nucleosomes recruit the CENP-A assembly factors CENP-C and M18BP1 independently. Furthermore, we demonstrate that the mechanism of CENP-C recruitment to centromeres is dependent on the density of underlying CENP-A nucleosomes.

PubMed ID: 26076692
PMC ID: PMC4477859
Article link: J Cell Biol
Grant support: [+]
Genes referenced: cenpa cenpc h4c4 mis18bp1 myc slc25a20

Article Images: [+] show captions
References [+] :
Bassett, HJURP uses distinct CENP-A surfaces to recognize and to stabilize CENP-A/histone H4 for centromere assembly. 2012, Pubmed

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