Fig. 1. Lateral view of the forebrain of Rana perezi. The letters on
the top refer to the levels of transverse sections shown in Figure 2.
Fig. 2. A–K: Series of transverse sections through the forebrain of
R. perezi. The far left vertical row presents high-contrast photomicrographs
of Nissl-stained sections in which the previously proposed
subdivisions of the basal telencephalon are indicated (after Northcutt
and Kicliter, 1980). In the second vertical row, the new subdivision, as
proposed on the basis of hodological (Marı´n et al., 1997a–d) and
chemoarchitectonic (present study) criteria, is depicted. The third
through sixth rows are line drawings at comparable levels showing the
distribution of tyrosine hydroxylase (TH)-, substance P (SP)-, enkephalin
(ENK)-, and nicotinamide adenine dinucleotide phosphate diaphorase
(NADPHd)-(immuno)reactive cell bodies (large dots) and fibers/
terminals (small dots, wavy lines). For abbreviations, see list.
Fig. 3. Photomicrographs illustrating TH-immunoreactive (THi)
fibers/terminals in the nucleus accumbens (A); in the striatum, ventral
pallidum, and bed nucleus of the stria terminalis (B); in the lateral
and anterior amygdaloid area (C); and in the medial and central
amygdala and the pallidal structures (D). Note the less dense innervation
of the dorsal part of the nucleus accumbens (Acc) compared with
its ventral part. The levels of the photomicrographs are comparable to
those of Figure 2C,E, and Figure 2E,G, respectively. For abbreviations,
see list. Scale bars 5 100 μm in A,C, 200 μm in B,D.
Fig. 4. A–C: Photomicrographs of transverse sections through
caudal telencephalic levels illustrating THi fibers/terminals seen in
the medial amygdala (MeA) and pallidal structures (A). The photomicrograph
in B shows the dense innervation of the periventricular zone
of the MeA in a cell-free zone, as seen in a Nissl-stained section (C).
Arrows indicate the lateral and medial boundaries of the periventricular
zone of the MeA. For abbreviations, see list. Scale bars 5 200 μm in
A, 100 μm in B,C.
Fig. 5. Photomicrographs of brain sections through the telencephalon
of R. perezi. Double-labeling techniques were used for SP (A–E),
calretinin (CR; F), choline acteyltransferase (ChAT; G), and neuropeptide
FF (NPFF; H, brown reaction) combined with NADPHd histochemistry
(blue reaction). A: Sagittal section illustrating the position of the
Acc and the bed nucleus of the stria terminalis (BST). B: Transverse
section showing SP innervation of the Acc, ventral pallidum (VP), and
AA as well as NADPHd cell bodies in the nucleus of the diagonal band
of Broca (DB) and the striatum (Str). C: High-magnification photomicrograph
of a transverse section through the lateral telencephalic wall
showing NADPHd reaction in the lateral amygdala (LA) and SP
immunoreactivity in the subjacent AA. D: Sagittal section illustrating
the relative position of the LA and the medial amygdala (MeA) at
caudal telencephalic levels (right side of the photomicrograph) and the
rostral position of the AA above the striatum up to the levels of the
accessory olfactory bulb. E: Photomicrograph of a transverse section at
caudal telencephalic levels showing the relative position of the central
amygdala (CeA), the striatopallidal system, and the BST. F: Sagittal
section at rostral telencephalic levels showing the relative distribution
of NADPHd and CR in the accessory bulb and in its caudal prolongation
above the striatum. G: Transverse section through caudal telencephalic
levels showing the distribution of NADPHdi and ChATi cells.
H: High-power photomicrograph showing NPFFi fibers in the Acc and
NADPHd activity in the surrounding regions. Arrows in B and F
indicate the caudal extent of the accessory olfactory bulb. For abbreviations,
see list. Scale bars 5 500 μm in A,D,F, 200 μm in B,E,G, 100 μm
Fig. 6. A–D: Photomicrographs of horizontal (A,C) and transverse (B,D) sections through the
telencephalon illustrating SPi fibers/terminals in the Acc and the BST (A) and in the MeA, BST, and
pallidal regions (B). ENKi fibers/terminals are shown in the BST and the dorsal pallidum (DP; C,D). Note
ENK1 cell bodies in the MeA. For abbreviations, see list. Scale bars 5 500 μm in A, 200 μm in B–D.
Fig. 7. Photomicrographs of transverse sections through the basal telencephalon illustrating NADPHdreactive
cell bodies and fibers at levels of the Acc (A), the ventral pallidum (B), and the CeA (C). Note the
absence of NADPHd reactivity in the Acc. For abbreviations, see list. Scale bars 5 200 μm in A,C, 150 μm
Fig. 8. Photomicrographs of transverse sections through the telencephalic
hemisphere of R. perezi showing CRi cell bodies and fibers/
terminals in the caudal part of the accessory bulb (A), in the AA just
caudal to the accessory olfactory bulb (B), and in the AA and Str at
midtelencephalic levels (C). Note the concise CRi fiber tract and
accompanying cells in theAA(B, arrow) and the diffuse labeling in the
striatal neuropil in C. For abbreviations, see list. Scale bars 5 200 μm
in A,C, 100 μm in B.
Fig. 9. Photomicrographs illustrating vasotocin (AVT)i cell bodies and fibers in the Acc (A) and
mesotocin (MST)1 neurons and fibers in the BST (B). For abbreviations, see list. Scale bars5 100 μm in A,
50 μm in B.
Fig. 10. Photomicrographs of transverse sections through the
rostral (A) and caudal (B) telencephalic levels showing dopamine
b-hydroxylase (DBH)i fibers/terminals in the VP, DP, and CeA. For
abbreviations, see list. Scale bars 5 100 μm.
Fig. 11. A–F: Photomicrographs of transverse sections through the
telencephalon illustrating somatostatin immunoreactive fibers/
terminals at rostral Acc and rostral Str levels (A), at midstriatal levels
(B), at levels of the CeA (C), and through the caudal pole of the MeA
(D). E and F show neuropeptide Y (NPY) staining in the transition
area between the AA and the MeA and in the caudal pole of the MeA,
respectively. For abbreviations, see list. Scale bars = 500 μm.