XB-ART-51369

J Biol Chem
November 13, 2015;
290
(46):
27557-71.
## Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells.

Abstract

A fundamental question in cell biology concerns the regulation of organelle size. While nuclear size is exquisitely controlled in different cell types, inappropriate nuclear enlargement is used to diagnose and stage cancer. Clarifying the functional significance of nuclear size necessitates an understanding of the mechanisms and proteins that control nuclear size. One structural component implicated in the regulation of nuclear morphology is the nuclear lamina, a meshwork of intermediate lamin filaments that lines the inner nuclear membrane. However, there has not been a systematic investigation of how the level and type of lamin expression influences nuclear size, in part due to difficulties in precisely controlling lamin expression levels in vivo. In this study, we circumvent this limitation by studying nuclei in Xenopus laevis egg and embryo extracts, open biochemical systems that allow for precise manipulation of lamin levels by the addition of recombinant proteins. We find that nuclear growth and size are sensitive to the levels of nuclear lamins, with low and high concentrations increasing and decreasing nuclear size, respectively. Interestingly, each type of lamin that we tested (lamins B1, B2, B3, and A) similarly affected nuclear size whether added alone or in combination, suggesting that total lamin concentration, and not lamin type, is more critical to determining nuclear size. Furthermore, we show that altering lamin levels in vivo, both in Xenopus embryos and mammalian tissue culture cells, also impacts nuclear size. These results have implications for normal development and carcinogenesis where both nuclear size and lamin expression levels change.

PubMed ID: 26429910

PMC ID: PMC4646008

Article link: J Biol Chem

Grant support: [+]

Genes referenced: ckap2 gnl3 h2bc21 igf2bp3 lmnb1 lmnb3 ran

Article Images: [+] show captions

References:

Abdalla, 2009, Pubmed
[-]

Adam, 2008, Pubmed, Xenbase

Agrelo, 2005, Pubmed

Alfonso, 2008, Pubmed

Anderson, 2007, Pubmed, Xenbase

Anderson, 2008, Pubmed

Benavente, 1985, Pubmed, Xenbase

Bengtsson, 2007, Pubmed

Broers, 1993, Pubmed

Capell, 2005, Pubmed

Chan, 2010, Pubmed

Chan, 2006, Pubmed, Xenbase

Chow, 2012, Pubmed

Coradeghini, 2006, Pubmed

Dahl, 2004, Pubmed, Xenbase

Davidson, 2014, Pubmed

Dittmer, 2011, Pubmed, Xenbase

Edens, 2014, Pubmed, Xenbase

Ellis, 1998, Pubmed, Xenbase

Foeger, 2006, Pubmed

Foster, 2010, Pubmed

Gieffers, 1991, Pubmed, Xenbase

Gu, 2004, Pubmed

Guo, 2014, Pubmed

Hara, 2015, Pubmed, Xenbase

Hatch, 2014, Pubmed

Heald, 1990, Pubmed

Heitlinger, 1991, Pubmed

Heitlinger, 1992, Pubmed

Hudson, 2007, Pubmed

Isobe, 2007, Pubmed

Izumi, 2000, Pubmed, Xenbase

Jenkins, 1994, Pubmed, Xenbase

Jevtić, 2014, Pubmed, Xenbase

Jevtić, 2015, Pubmed, Xenbase

Jung, 2014, Pubmed

Karabinos, 2002, Pubmed

Kau, 2004, Pubmed

Kaufmann, 1991, Pubmed

Kitten, 1991, Pubmed

Kochin, 2014, Pubmed

Kuramitsu, 2010, Pubmed

Lammerding, 2004, Pubmed

Lammerding, 2006, Pubmed

Levy, 2010, Pubmed, Xenbase

Levy, 2012, Pubmed, Xenbase

Lim, 2002, Pubmed

Mall, 2012, Pubmed

Mallampalli, 2005, Pubmed

Moss, 1999, Pubmed

Mossbacher, 1996, Pubmed

Newport, 1991, Pubmed, Xenbase

Oguchi, 2002, Pubmed

Paradisi, 2005, Pubmed

Prüfert, 2004, Pubmed, Xenbase

Prüfert, 2005, Pubmed, Xenbase

Ralle, 2004, Pubmed, Xenbase

Roth, 2010, Pubmed

Schirmer, 2001, Pubmed

Schirmer, 2004, Pubmed

Shimi, 2014, Pubmed

Shimi, 2009, Pubmed

Shumaker, 2005, Pubmed, Xenbase

Skvortsov, 2011, Pubmed

Stick, 1985, Pubmed, Xenbase

Sun, 2014, Pubmed, Xenbase

Sun, 2010, Pubmed

Venables, 2001, Pubmed

Wang, 1992, Pubmed

Wang, 2009, Pubmed

Wilson, 2010, Pubmed

Wu, 2009, Pubmed

Wühr, 2014, Pubmed, Xenbase

Yanai, 2011, Pubmed, Xenbase

Zink, 2004, Pubmed

Adam, 2008, Pubmed, Xenbase

Agrelo, 2005, Pubmed

Alfonso, 2008, Pubmed

Anderson, 2007, Pubmed, Xenbase

Anderson, 2008, Pubmed

Benavente, 1985, Pubmed, Xenbase

Bengtsson, 2007, Pubmed

Broers, 1993, Pubmed

Capell, 2005, Pubmed

Chan, 2010, Pubmed

Chan, 2006, Pubmed, Xenbase

Chow, 2012, Pubmed

Coradeghini, 2006, Pubmed

Dahl, 2004, Pubmed, Xenbase

Davidson, 2014, Pubmed

Dittmer, 2011, Pubmed, Xenbase

Edens, 2014, Pubmed, Xenbase

Ellis, 1998, Pubmed, Xenbase

Foeger, 2006, Pubmed

Foster, 2010, Pubmed

Gieffers, 1991, Pubmed, Xenbase

Gu, 2004, Pubmed

Guo, 2014, Pubmed

Hara, 2015, Pubmed, Xenbase

Hatch, 2014, Pubmed

Heald, 1990, Pubmed

Heitlinger, 1991, Pubmed

Heitlinger, 1992, Pubmed

Hudson, 2007, Pubmed

Isobe, 2007, Pubmed

Izumi, 2000, Pubmed, Xenbase

Jenkins, 1994, Pubmed, Xenbase

Jevtić, 2014, Pubmed, Xenbase

Jevtić, 2015, Pubmed, Xenbase

Jung, 2014, Pubmed

Karabinos, 2002, Pubmed

Kau, 2004, Pubmed

Kaufmann, 1991, Pubmed

Kitten, 1991, Pubmed

Kochin, 2014, Pubmed

Kuramitsu, 2010, Pubmed

Lammerding, 2004, Pubmed

Lammerding, 2006, Pubmed

Levy, 2010, Pubmed, Xenbase

Levy, 2012, Pubmed, Xenbase

Lim, 2002, Pubmed

Mall, 2012, Pubmed

Mallampalli, 2005, Pubmed

Moss, 1999, Pubmed

Mossbacher, 1996, Pubmed

Newport, 1991, Pubmed, Xenbase

Oguchi, 2002, Pubmed

Paradisi, 2005, Pubmed

Prüfert, 2004, Pubmed, Xenbase

Prüfert, 2005, Pubmed, Xenbase

Ralle, 2004, Pubmed, Xenbase

Roth, 2010, Pubmed

Schirmer, 2001, Pubmed

Schirmer, 2004, Pubmed

Shimi, 2014, Pubmed

Shimi, 2009, Pubmed

Shumaker, 2005, Pubmed, Xenbase

Skvortsov, 2011, Pubmed

Stick, 1985, Pubmed, Xenbase

Sun, 2014, Pubmed, Xenbase

Sun, 2010, Pubmed

Venables, 2001, Pubmed

Wang, 1992, Pubmed

Wang, 2009, Pubmed

Wilson, 2010, Pubmed

Wu, 2009, Pubmed

Wühr, 2014, Pubmed, Xenbase

Yanai, 2011, Pubmed, Xenbase

Zink, 2004, Pubmed