Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-51462
PLoS One. January 1, 2015; 10 (10): e0138313.

A Database of microRNA Expression Patterns in Xenopus laevis.

Ahmed A , Ward NJ , Moxon S , Lopez-Gomollon S , Viaut C , Tomlinson ML , Patrushev I , Gilchrist MJ , Dalmay T , Dotlic D , Münsterberg AE , Wheeler GN .


Abstract
MicroRNAs (miRNAs) are short, non-coding RNAs around 22 nucleotides long. They inhibit gene expression either by translational repression or by causing the degradation of the mRNAs they bind to. Many are highly conserved amongst diverse organisms and have restricted spatio-temporal expression patterns during embryonic development where they are thought to be involved in generating accuracy of developmental timing and in supporting cell fate decisions and tissue identity. We determined the expression patterns of 180 miRNAs in Xenopus laevis embryos using LNA oligonucleotides. In addition we carried out small RNA-seq on different stages of early Xenopus development, identified 44 miRNAs belonging to 29 new families and characterized the expression of 5 of these. Our analyses identified miRNA expression in many organs of the developing embryo. In particular a large number were expressed in neural tissue and in the somites. Surprisingly none of the miRNAs we have looked at show expression in the heart. Our results have been made freely available as a resource in both XenMARK and Xenbase.

PubMed ID: 26506012
PMC ID: PMC4624429
Article link: PLoS One.
Grant support: BB/D016444/1 Biotechnology and Biological Sciences Research Council , BB/H003525/1 Biotechnology and Biological Sciences Research Council , BB/H003525 Biotechnology and Biological Sciences Research Council , Cancer Research UK, BB_BB/D016444/1 Biotechnology and Biological Sciences Research Council , BB_BB/H003525/1 Biotechnology and Biological Sciences Research Council , BB/H003525 Biotechnology and Biological Sciences Research Council , Cancer Research UK, BB/D016444/1 Biotechnology and Biological Sciences Research Council , BB/H003525/1 Biotechnology and Biological Sciences Research Council , BB/H003525 Biotechnology and Biological Sciences Research Council , Cancer Research UK



References:
Agrawal, 2009, Pubmed, Xenbase[+]


Article Images: [+] show captions

My Xenbase: [ Log-in / Register ]
version: [4.5.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556