Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-51474
PLoS One January 1, 2015; 10 (10): e0139504.

Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish.

Pauli A , Montague TG , Lennox KA , Behlke MA , Schier AF .


Abstract
Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H. ASOs are widely used as gene knockdown reagents in tissue culture and in Xenopus and mouse model systems. To test their effectiveness in zebrafish, we targeted 20 developmental genes and compared the morphological changes with mutant and morpholino (MO)-induced phenotypes. ASO-mediated transcript knockdown reproduced the published loss-of-function phenotypes for oep, chordin, dnd, ctnnb2, bmp7a, alk8, smad2 and smad5 in a dosage-sensitive manner. ASOs knocked down both maternal and zygotic transcripts, as well as the long noncoding RNA (lncRNA) MALAT1. ASOs were only effective within a narrow concentration range and were toxic at higher concentrations. Despite this drawback, quantitation of knockdown efficiency and the ability to degrade lncRNAs make ASOs a useful knockdown reagent in zebrafish.

PubMed ID: 26436892
PMC ID: PMC4593562
Article link: PLoS One
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: acvr1 bmp1 chrd.1 dnd1 eef1a2 pnhd smad2 tp53 wnt11 wnt5b


Article Images: [+] show captions
References [+] :
Bauer, The type I serine/threonine kinase receptor Alk8/Lost-a-fin is required for Bmp2b/7 signal transduction during dorsoventral patterning of the zebrafish embryo. 2001, Pubmed