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XB-ART-51690
Sci Rep September 21, 2015; 5 17893.

Ca-α1T, a fly T-type Ca2+ channel, negatively modulates sleep.

Jeong K , Lee S , Seo H , Oh Y , Jang D , Choe J , Kim D , Lee JH , Jones WD .


Abstract
Mammalian T-type Ca(2+) channels are encoded by three separate genes (Cav3.1, 3.2, 3.3). These channels are reported to be sleep stabilizers important in the generation of the delta rhythms of deep sleep, but controversy remains. The identification of precise physiological functions for the T-type channels has been hindered, at least in part, by the potential for compensation between the products of these three genes and a lack of specific pharmacological inhibitors. Invertebrates have only one T-type channel gene, but its functions are even less well-studied. We cloned Ca-α1T, the only Cav3 channel gene in Drosophila melanogaster, expressed it in Xenopus oocytes and HEK-293 cells, and confirmed it passes typical T-type currents. Voltage-clamp analysis revealed the biophysical properties of Ca-α1T show mixed similarity, sometimes falling closer to Cav3.1, sometimes to Cav3.2, and sometimes to Cav3.3. We found Ca-α1T is broadly expressed across the adult fly brain in a pattern vaguely reminiscent of mammalian T-type channels. In addition, flies lacking Ca-α1T show an abnormal increase in sleep duration most pronounced during subjective day under continuous dark conditions despite normal oscillations of the circadian clock. Thus, our study suggests invertebrate T-type Ca(2+) channels promote wakefulness rather than stabilizing sleep.

PubMed ID: 26647714
PMC ID: PMC4673464
Article link: Sci Rep

Genes referenced: cacna1g cacna1h cacna1i cav3.1 cav3.2 clock cnga1 gnl3 igf2bp3


Article Images: [+] show captions
References:
Alekseyenko, 2010, Pubmed [+]


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