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XB-ART-51735
Elife. March 18, 2014; 3 e01439.

Coordinated genomic control of ciliogenesis and cell movement by RFX2.



Abstract
The mechanisms linking systems-level programs of gene expression to discrete cell biological processes in vivo remain poorly understood. In this study, we have defined such a program for multi-ciliated epithelial cells (MCCs), a cell type critical for proper development and homeostasis of the airway, brain and reproductive tracts. Starting from genomic analysis of the cilia-associated transcription factor Rfx2, we used bioinformatics and in vivo cell biological approaches to gain insights into the molecular basis of cilia assembly and function. Moreover, we discovered a previously un-recognized role for an Rfx factor in cell movement, finding that Rfx2 cell-autonomously controls apical surface expansion in nascent MCCs. Thus, Rfx2 coordinates multiple, distinct gene expression programs in MCCs, regulating genes that control cell movement, ciliogenesis, and cilia function. As such, the work serves as a paradigm for understanding genomic control of cell biological processes that span from early cell morphogenetic events to terminally differentiated cellular functions. DOI: http://dx.doi.org/10.7554/eLife.01439.001.

PubMed ID: 24424412
PMC ID: PMC3889689
Article link: Elife.
Grant support: P40 OD010997 NIH HHS , R01 GM074104 NIGMS NIH HHS , R01 HL117164 NHLBI NIH HHS , R01 GM074104 NIGMS NIH HHS , R01 HD076839 NICHD NIH HHS , R01 HL117164 NHLBI NIH HHS

Genes referenced: act3 ccdc63 cfap36 dnal1 foxj1.2 ift172 ift20 kidins220 mcc mns1 nme5 pacrg rfx2 ribc2 ropn1l slit2 tbx2 tekt3 ttc29 tuba4a utrn tuba4b

Antibodies referenced: GFP Ab15 RFP Ab3 Tuba4b Ab2 Tuba4b Ab4
Morpholinos referenced: rfx2 MO1 ribc2 MO1 slit2 MO1 ttc29 MO1

References:
Afzelius, 1976, Pubmed[+]


Article Images: [+] show captions

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