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XB-ART-51735
Elife January 1, 2014; 3 e01439.

Coordinated genomic control of ciliogenesis and cell movement by RFX2.



Abstract
The mechanisms linking systems-level programs of gene expression to discrete cell biological processes in vivo remain poorly understood. In this study, we have defined such a program for multi-ciliated epithelial cells (MCCs), a cell type critical for proper development and homeostasis of the airway, brain and reproductive tracts. Starting from genomic analysis of the cilia-associated transcription factor Rfx2, we used bioinformatics and in vivo cell biological approaches to gain insights into the molecular basis of cilia assembly and function. Moreover, we discovered a previously un-recognized role for an Rfx factor in cell movement, finding that Rfx2 cell-autonomously controls apical surface expansion in nascent MCCs. Thus, Rfx2 coordinates multiple, distinct gene expression programs in MCCs, regulating genes that control cell movement, ciliogenesis, and cilia function. As such, the work serves as a paradigm for understanding genomic control of cell biological processes that span from early cell morphogenetic events to terminally differentiated cellular functions. DOI: http://dx.doi.org/10.7554/eLife.01439.001.

PubMed ID: 24424412
PMC ID: PMC3889689
Article link: Elife
Grant support: [+]
Genes referenced: tuba4b tuba8 ropn1l dnal1 cfap36 tekt3 ttc29 ribc2 ccdc63 acta4 foxj1.2 ift172 ift20 kidins220 mcc mcidas mns1 nme5 pacrg rfx2 slit2 tbx2 tubal3.1 utrn
Antibodies: GFP Ab15 RFP Ab3 Tuba4b Ab2 Tuba4b Ab4
Morpholinos: rfx2 MO1 ribc2 MO1 slit2 MO1 ttc29 MO1


Article Images: [+] show captions
References:
Afzelius, 1976, Pubmed [+]


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