Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Neurotoxicology
2017 May 01;60:142-149. doi: 10.1016/j.neuro.2016.03.010.
Show Gene links
Show Anatomy links
Functional reconstitution of rat Nav1.6 sodium channels in vitro for studies of pyrethroid action.
Soderlund DM
,
Tan J
,
He B
.
???displayArticle.abstract???
The ability to reconstitute sodium channel function and pharmacology in vitro using cloned subunits of known structure has greatly enhanced our understanding of the action of pyrethroid insecticides at this target and the structural determinants of resistance and interspecies selectivity. However, the use of reconstituted channels raises three critical questions: (1) Which subunits and subunit combinations should be used? (2) Which heterologous expression system is preferred? (3) Which combination of subunits and expression system best represents the function of native neuronal channels in the organism of interest? This review considers these questions from the perspective of recent research in this laboratory on the action of pyrethroid insecticides on rat Nav1.6 sodium channels by comparing the effects of heteroligomeric complex composition on channel function and insecticide response when channels are expressed in either Xenopus oocytes or stably-transformed HEK293 cells. These comparisons provide new insight into the influence of cellular context on the functional and pharmacological properties of expressed channels, the modulatory effects of sodium channel auxiliary subunits on the action of pyrethroids, and the relative fidelity of the Xenopus oocyte and HEK293 cell expression systems as model systems for studying of channel function and pyrethroid action.
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988, Pubmed,
Xenbase
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988,
Pubmed
,
Xenbase
Berjukow,
Endogenous calcium channels in human embryonic kidney (HEK293) cells.
1996,
Pubmed
Caldwell,
Sodium channel Na(v)1.6 is localized at nodes of ranvier, dendrites, and synapses.
2000,
Pubmed
Choi,
Structure-activity relationships for the action of 11 pyrethroid insecticides on rat Na v 1.8 sodium channels expressed in Xenopus oocytes.
2006,
Pubmed
,
Xenbase
Dalhoff,
Regulation of sulphation and glutathion conjugation of acetaminophen in isolated rat hepatocytes.
1996,
Pubmed
Felts,
Sodium channel alpha-subunit mRNAs I, II, III, NaG, Na6 and hNE (PN1): different expression patterns in developing rat nervous system.
1997,
Pubmed
Ginsburg,
Differential sensitivity of tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels to the insecticide allethrin in rat dorsal root ganglion neurons.
1993,
Pubmed
Goldin,
Resurgence of sodium channel research.
2001,
Pubmed
Goldin,
Evolution of voltage-gated Na(+) channels.
2002,
Pubmed
Graham,
Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
1977,
Pubmed
Harrill,
Time and concentration dependent accumulation of [3H]-deltamethrin in Xenopus laevis oocytes.
2005,
Pubmed
,
Xenbase
Hartshorne,
The sodium channel from rat brain. Purification and subunit composition.
1984,
Pubmed
He,
Differential state-dependent modification of rat Na(v)1.6 sodium channels expressed in human embryonic kidney (HEK293) cells by the pyrethroid insecticides tefluthrin and deltamethrin.
2011,
Pubmed
,
Xenbase
He,
Human embryonic kidney (HEK293) cells express endogenous voltage-gated sodium currents and Na v 1.7 sodium channels.
2010,
Pubmed
He,
Effects of the β1 auxiliary subunit on modification of Rat Na(v)1.6 sodium channels expressed in HEK293 cells by the pyrethroid insecticides tefluthrin and deltamethrin.
2016,
Pubmed
He,
Functional expression of Rat Nav1.6 voltage-gated sodium channels in HEK293 cells: modulation by the auxiliary β1 subunit.
2014,
Pubmed
,
Xenbase
Hu,
Distinct contributions of Na(v)1.6 and Na(v)1.2 in action potential initiation and backpropagation.
2009,
Pubmed
Isom,
Sodium channel beta subunits: anything but auxiliary.
2001,
Pubmed
Jiang,
Endogenous Kv channels in human embryonic kidney (HEK-293) cells.
2002,
Pubmed
Meacham,
Developmentally-regulated sodium channel subunits are differentially sensitive to alpha-cyano containing pyrethroids.
2008,
Pubmed
,
Xenbase
Narahashi,
Neuronal ion channels as the target sites of insecticides.
1996,
Pubmed
Rinkevich,
Diversity and Convergence of Sodium Channel Mutations Involved in Resistance to Pyrethroids.
2013,
Pubmed
Schaller,
Expression and distribution of voltage-gated sodium channels in the cerebellum.
2003,
Pubmed
Shah,
Developmental expression of the novel voltage-gated sodium channel auxiliary subunit beta3, in rat CNS.
2001,
Pubmed
,
Xenbase
Shaw,
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
2002,
Pubmed
Smith,
Action of the pyrethroid insecticide cypermethrin on rat brain IIa sodium channels expressed in xenopus oocytes.
1998,
Pubmed
,
Xenbase
Soderlun,
Point mutations in homology domain II modify the sensitivity of rat Nav1.8 sodium channels to the pyrethroid insecticide cismethrin.
2001,
Pubmed
,
Xenbase
Soderlund,
The molecular biology of knockdown resistance to pyrethroid insecticides.
2003,
Pubmed
Soderlund,
State-Dependent Modification of Voltage-Gated Sodium Channels by Pyrethroids.
2010,
Pubmed
,
Xenbase
Song,
Interactions of tetramethrin, fenvalerate and DDT at the sodium channel in rat dorsal root ganglion neurons.
1996,
Pubmed
Tabarean,
Potent modulation of tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels by the type II pyrethroid deltamethrin.
1998,
Pubmed
Tan,
Human and rat Nav1.3 voltage-gated sodium channels differ in inactivation properties and sensitivity to the pyrethroid insecticide tefluthrin.
2009,
Pubmed
,
Xenbase
Tan,
Actions of Tefluthrin on Rat Na(v)1.7 Voltage-Gated Sodium Channels Expressed in Xenopus Oocytes.
2011,
Pubmed
,
Xenbase
Tan,
Coexpression with Auxiliary β Subunits Modulates the Action of Tefluthrin on Rat Na(v)1.6 and Na(v)1.3 Sodium Channels.
2011,
Pubmed
,
Xenbase
Tan,
Divergent actions of the pyrethroid insecticides S-bioallethrin, tefluthrin, and deltamethrin on rat Na(v)1.6 sodium channels.
2010,
Pubmed
,
Xenbase
Tatebayashi,
Differential mechanism of action of the pyrethroid tetramethrin on tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels.
1994,
Pubmed
Thomas,
HEK293 cell line: a vehicle for the expression of recombinant proteins.
2005,
Pubmed
Vais,
A single amino acid change makes a rat neuronal sodium channel highly sensitive to pyrethroid insecticides.
2000,
Pubmed
,
Xenbase
Whitaker,
Comparative distribution of voltage-gated sodium channel proteins in human brain.
2001,
Pubmed
Whitaker,
Distribution of voltage-gated sodium channel alpha-subunit and beta-subunit mRNAs in human hippocampal formation, cortex, and cerebellum.
2000,
Pubmed
Wu,
Underlying mechanism of actions of tefluthrin, a pyrethroid insecticide, on voltage-gated ion currents and on action currents in pituitary tumor (GH3) cells and GnRH-secreting (GT1-7) neurons.
2009,
Pubmed
