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XB-ART-52020
Sci Rep. September 28, 2016; 6 24104.

Prickle3 synergizes with Wtip to regulate basal body organization and cilia growth.

Chu CW , Ossipova O , Ioannou A , Sokol SY .


Abstract
PCP proteins maintain planar polarity in many epithelial tissues and have been implicated in cilia development in vertebrate embryos. In this study we examine Prickle3 (Pk3), a vertebrate homologue of Drosophila Prickle, in Xenopus gastrocoel roof plate (GRP). GRP is a tissue equivalent to the mouse node, in which cilia-generated flow promotes left-right patterning. We show that Pk3 is enriched at the basal body of GRP cells but is recruited by Vangl2 to anterior cell borders. Interference with Pk3 function disrupted the anterior polarization of endogenous Vangl2 and the posterior localization of cilia in GRP cells, demonstrating its role in PCP. Strikingly, in cells with reduced Pk3 activity, cilia growth was inhibited and γ-tubulin and Nedd1 no longer associated with the basal body, suggesting that Pk3 has a novel function in basal body organization. Mechanistically, this function of Pk3 may involve Wilms tumor protein 1-interacting protein (Wtip), which physically associates with and cooperates with Pk3 to regulate ciliogenesis. We propose that, in addition to cell polarity, PCP components control basal body organization and function.

PubMed ID: 27062996
PMC ID: PMC4827067
Article link: Sci Rep.

Genes referenced: prickle1 prickle3 tub wtip

Morpholinos referenced: Prickle3 MO1 Temporary prickle3 MO1 prickle3 MO2 wtip MO2 wtip MO3

References:
Ambegaonkar, 2016, Pubmed[+]


Article Images: [+] show captions

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