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Dev Biol January 1, 2017; 426 (2): 165-175.

Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

Kjolby RAS , Harland RM .

The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach. We selected putative targets using the criteria of reduced expression upon zygotic Wnt knockdown, β-catenin binding within 50kb of the gene, and expression in tissues that receive Wnt signaling. Using these criteria, we found 21 novel direct transcriptional targets of Wnt/β-catenin signaling during gastrulation and in addition have identified putative regulatory elements for further characterization in future studies.

PubMed ID: 27091726
PMC ID: PMC6288011
Article link: Dev Biol
Grant support: [+]
Genes referenced: axin2 ccn1l cdon cdx1 cdx2 ctnnb1 dkk1 esr-5 fzd10 gbx2.1 gbx2.2 hes5.2 hes5.7 hes5.8 hes5.9 hes6.1 hoxa1 irx3 kremen2 msgn1 mtx2 myo3b neurog2 ngfr pax3 pnhd sp5 tacc1 tcf7 tp63 vegt wnt8a zadh2 znf703
GO keywords: gastrulation [+]

GEO Series:
   GSE77363: NCBI
   GSE77364: NCBI
   GSE77365: NCBI

Article Images: [+] show captions
References [+] :
Anders, HTSeq--a Python framework to work with high-throughput sequencing data. 2015, Pubmed

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