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XB-ART-52045
Development January 1, 2016; 143 (12): 2194-205.

Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by Dact1/2.

Rabadán MA , Herrera A , Fanlo L , Usieto S , Carmona-Fontaine C , Barriga EH , Mayor R , Pons S , Martí E .


Abstract
Delamination of neural crest (NC) cells is a bona fide physiological model of epithelial-to-mesenchymal transition (EMT), a process that is influenced by Wnt/β-catenin signalling. Using two in vivo models, we show that Wnt/β-catenin signalling is transiently inhibited at the time of NC delamination. In attempting to define the mechanism underlying this inhibition, we found that the scaffold proteins Dact1 and Dact2, which are expressed in pre-migratory NC cells, are required for NC delamination in Xenopus and chick embryos, whereas they do not affect the motile properties of migratory NC cells. Dact1/2 inhibit Wnt/β-catenin signalling upstream of the transcriptional activity of T cell factor (TCF), which is required for EMT to proceed. Dact1/2 regulate the subcellular distribution of β-catenin, preventing β-catenin from acting as a transcriptional co-activator to TCF, yet without affecting its stability. Together, these data identify a novel yet important regulatory element that inhibits β-catenin signalling, which then affects NC delamination.

PubMed ID: 27122165
PMC ID: PMC4920176
Article link: Development
Grant support: [+]
Genes referenced: ctnnb1 dact1 itk nts sox2 twist1
Morpholinos: dact1 MO2


Article Images: [+] show captions
References [+] :
Alvares, Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes. 2009, Pubmed


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