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XB-ART-52086
Dev Dyn January 1, 2016; 245 (8): 854-73.

CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.

Blech-Hermoni Y , Sullivan CB , Jenkins MW , Wessely O , Ladd AN .


Abstract
BACKGROUND: CUG-BP, Elav-like family member 1 (CELF1) is a multifunctional RNA binding protein found in a variety of adult and embryonic tissues. In the heart, CELF1 is found exclusively in the myocardium. However, the roles of CELF1 during cardiac development have not been completely elucidated. RESULTS: Myofibrillar organization is disrupted and proliferation is reduced following knockdown of CELF1 in cultured chicken primary embryonic cardiomyocytes. In vivo knockdown of Celf1 in developing Xenopus laevis embryos resulted in myofibrillar disorganization and a trend toward reduced proliferation in heart muscle, indicating conserved roles for CELF1 orthologs in embryonic cardiomyocytes. Loss of Celf1 also resulted in morphogenetic abnormalities in the developing heart and gut. Using optical coherence tomography, we showed that cardiac contraction was impaired following depletion of Celf1, while heart rhythm remained unperturbed. In contrast to cardiac muscle, loss of Celf1 did not disrupt myofibril organization in skeletal muscle cells, although it did lead to fragmentation of skeletal muscle bundles. CONCLUSIONS: CELF1 is required for normal myofibril organization, proliferation, morphogenesis, and contractile performance in the developing myocardium. Developmental Dynamics 245:854-873, 2016. © 2016 Wiley Periodicals, Inc.

PubMed ID: 27144987
PMC ID: PMC4946975
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actn2 ccnd1 ccnd2 cdkn1a cdkn1b celf1 gapdh gata4 hand1 hand2 nkx2-5 ttn tubg1
Antibodies: Celf1 Ab2
Morpholinos: celf1 MO1 celf1 MO2

Phenotypes: Xla Wt + celf1 MO (Fig.10 A, B) [+]

Article Images: [+] show captions
References [+] :
Baldwin, Epidermal growth factor receptor stimulation activates the RNA binding protein CUG-BP1 and increases expression of C/EBPbeta-LIP in mammary epithelial cells. 2004, Pubmed