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Cell Rep January 1, 2016; 15 (8): 1624-33.

Centromeric Transcription Regulates Aurora-B Localization and Activation.

Centromeric transcription is widely conserved; however, it is not clear what role centromere transcription plays during mitosis. Here, I find that centromeres are transcribed in Xenopus egg extracts into a long noncoding RNA (lncRNA; cen-RNA) that localizes to mitotic centromeres, chromatin, and spindles. cen-RNAs bind to the chromosomal passenger complex (CPC) in vitro and in vivo. Blocking transcription or antisense inhibition of cen-RNA leads to a reduction of CPC localization to the inner centromere and misregulation of CPC component Aurora-B activation independently of known centromere recruitment pathways. Additionally, transcription is required for normal bipolar attachment of kinetochores to the mitotic spindle, consistent with a role for cen-RNA in CPC regulation. This work demonstrates that cen-RNAs promote normal kinetochore function through regulation of the localization and activation of the CPC and confirm that lncRNAs are components of the centromere.

PubMed ID: 27184843
PMC ID: PMC4880503
Article link: Cell Rep
Grant support: [+]
Genes referenced: bub1 cdca9 frrs1 tff3.1
GO keywords: kinetochore assembly [+]
Antibodies: Polr2a Ab5

Article Images: [+] show captions
References [+] :
Andrews, Aurora B regulates MCAK at the mitotic centromere. 2004, Pubmed

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