XB-ART-52277
Dev Genes Evol
January 1, 2016;
226
(5):
369-82.
Comparative expression study of sipa family members during early Xenopus laevis development.
Rothe M
,
Monteiro F
,
Dietmann P
,
Kühl SJ
.
The signal-induced proliferation-associated (SIPA) protein family belongs to the
RapGAP protein superfamily. Previous studies mainly focused on the expression and function of SIPA genes in vertebrate neuronal
tissue. Only limited data about the embryonic expression pattern of the genes are currently available. Our study provides the first expression analysis of
sipa1,
sipa1l1,
sipa1l2, and
sipa1l3 during early development of the vertebrate organism Xenopus laevis. In silico, analysis revealed that all genes are highly conserved across species. Semi-quantitative
RT-PCR experiments demonstrated that the RNA of all genes was maternally supplied. By whole mount in situ hybridization approaches, we showed that
sipa1 is mainly expressed in various sensory organs, the respiratory and
blood system,
heart,
neural tube, and
eye. In contrast,
sipa1l1 showed a broad expression during development in particular within the
brain,
somites,
eye, and
heart.
Sipa1l2 was detected in the
branchial arches,
glomerulus, and the developing
eye. In contrast,
sipa1l3 revealed a
tissue specific expression within the olfactory and otic vesicles, the
cranial placodes and
ganglia,
neural tube,
pronephros,
retina, and
lens. In summary, all sipa gene family members are expressed throughout the whole developing Xenopus organism and might play an important role during vertebrate early embryogenesis.
PubMed ID:
27384056
Article link:
Dev Genes Evol
Species referenced:
Xenopus laevis
Genes referenced:
rap1gap
sipa1
sipa1l1
sipa1l2
sipa1l3
References [+] :
Dolnik,
Sipa1l3/SPAR3 is targeted to postsynaptic specializations and interacts with the Fezzin ProSAPiP1/Lzts3.
2015,
Pubmed
Dolnik,
Sipa1l3/SPAR3 is targeted to postsynaptic specializations and interacts with the Fezzin ProSAPiP1/Lzts3.
2015,
Pubmed
Evers,
SIPA1L3 identified by linkage analysis and whole-exome sequencing as a novel gene for autosomal recessive congenital cataract.
2015,
Pubmed
Gao,
The E6 oncoproteins of high-risk papillomaviruses bind to a novel putative GAP protein, E6TP1, and target it for degradation.
1999,
Pubmed
Gdowicz-Kłosok,
The SIPA1 -313A>G polymorphism is associated with prognosis in inoperable non-small cell lung cancer.
2015,
Pubmed
Greenlees,
Mutations in SIPA1L3 cause eye defects through disruption of cell polarity and cytoskeleton organization.
2015,
Pubmed
Hattori,
Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression.
1995,
Pubmed
Hemmati-Brivanlou,
Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization.
1992,
Pubmed
,
Xenbase
Herrick,
Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage.
2010,
Pubmed
Ji,
Expression of signal-induced proliferation-associated gene 1 (SIPA1), a RapGTPase-activating protein, is increased in colorectal cancer and has diverse effects on functions of colorectal cancer cells.
2012,
Pubmed
Lachke,
iSyTE: integrated Systems Tool for Eye gene discovery.
2012,
Pubmed
Letunic,
Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees.
2016,
Pubmed
Mitchell,
The InterPro protein families database: the classification resource after 15 years.
2015,
Pubmed
Nagase,
Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
2001,
Pubmed
Pak,
Regulation of dendritic spine morphology by SPAR, a PSD-95-associated RapGAP.
2001,
Pubmed
Sievers,
Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.
2011,
Pubmed
Spilker,
SPAR2, a novel SPAR-related protein with GAP activity for Rap1 and Rap2.
2008,
Pubmed
Spilker,
RapGAPs in brain: multipurpose players in neuronal Rap signalling.
2010,
Pubmed
Tsai,
A Wnt-CKIvarepsilon-Rap1 pathway regulates gastrulation by modulating SIPA1L1, a Rap GTPase activating protein.
2007,
Pubmed
,
Xenbase
Zhang,
Nuclear SIPA1 activates integrin β1 promoter and promotes invasion of breast cancer cells.
2015,
Pubmed
