XB-ART-52277
Dev Genes Evol
January 1, 2016;
226
(5):
369-82.
Comparative expression study of sipa family members during early Xenopus laevis development.
Rothe M
,
Monteiro F
,
Dietmann P
,
Kühl SJ
.
Abstract
The signal-induced proliferation-associated (SIPA) protein family belongs to the
RapGAP protein superfamily. Previous studies mainly focused on the expression and function of SIPA genes in vertebrate neuronal
tissue. Only limited data about the embryonic expression pattern of the genes are currently available. Our study provides the first expression analysis of
sipa1,
sipa1l1,
sipa1l2, and
sipa1l3 during early development of the vertebrate organism Xenopus laevis. In silico, analysis revealed that all genes are highly conserved across species. Semi-quantitative
RT-PCR experiments demonstrated that the RNA of all genes was maternally supplied. By whole mount in situ hybridization approaches, we showed that
sipa1 is mainly expressed in various sensory organs, the respiratory and
blood system,
heart,
neural tube, and
eye. In contrast,
sipa1l1 showed a broad expression during development in particular within the
brain,
somites,
eye, and
heart.
Sipa1l2 was detected in the
branchial arches,
glomerulus, and the developing
eye. In contrast,
sipa1l3 revealed a
tissue specific expression within the olfactory and otic vesicles, the
cranial placodes and
ganglia,
neural tube,
pronephros,
retina, and
lens. In summary, all sipa gene family members are expressed throughout the whole developing Xenopus organism and might play an important role during vertebrate early embryogenesis.
PubMed ID:
27384056
Article link:
Dev Genes Evol
Species referenced:
Xenopus laevis
Genes referenced:
rap1gap
sipa1
sipa1l1
sipa1l2
sipa1l3
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