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XB-ART-52308
Biol Open September 15, 2016; 5 (9): 1275-82.

Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages.

Tlapakova T , Nguyen TM , Vegrichtova M , Sidova M , Strnadova K , Blahova M , Krylov V .


Abstract
The origin of somatic cell lineages during testicular development is controversial in mammals. Employing basal amphibian tetrapod Xenopus tropicalis we established a cell culture derived from testes of juvenile male. Expression analysis showed transcription of some pluripotency genes and Sertoli cell, peritubular myoid cell and mesenchymal cell markers. Transcription of germline-specific genes was downregulated. Immunocytochemistry revealed that a majority of cells express vimentin and co-express Sox9 and smooth muscle α-actin (Sma), indicating the existence of a common progenitor of Sertoli and peritubular myoid cell lineages. Microinjection of transgenic, red fluorescent protein (RFP)-positive somatic testicular cells into the peritoneal cavity of X. tropicalis tadpoles resulted in cell deposits in heart, pronephros and intestine, and later in a strong proliferation and formation of cell-to-cell net growing through the tadpole body. Immunohistochemistry analysis of transplanted tadpoles showed a strong expression of vimentin in RFP-positive cells. No co-localization of Sox9 and Sma signals was observed during the first three weeks indicating their dedifferentiation to migratory-active mesenchymal cells recently described in human testicular biopsies.

PubMed ID: 27464670
PMC ID: PMC5051652
Article link: Biol Open

Genes referenced: acta2 cd44 cyp11a1 cyp17a1 dazl ddx25 ddx4 itgb1 kitlg lif odc1 sox9 thy1 vim
Antibodies: Acta2 Ab1 Sox9 Ab1 Vim Ab1


Article Images: [+] show captions
References:
Abe, 2007, Pubmed [+]


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