Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In Vitro Cell Dev Biol Anim
2017 Mar 01;533:231-247. doi: 10.1007/s11626-016-0099-9.
Show Gene links
Show Anatomy links
Insulin-like growth factor 1 regulation of proliferation and differentiation of Xenopus laevis myogenic cells in vitro.
Miyata S
,
Yada T
,
Ishikawa N
,
Taheruzzaman K
,
Hara R
,
Matsuzaki T
,
Nishikawa A
.
???displayArticle.abstract???
To understand the mechanism of muscle remodeling during Xenopus laevis metamorphosis, we examined the in vitro effect of insulin-like growth factor 1 (IGF-1) on growth and differentiation of three different-fate myogenic cell populations: tadpole tail, tadpole dorsal, and young adult leg muscle. IGF-1 promoted growth and differentiation of both tail and leg myogenic cells only under conditions where these cells could proliferate. Inhibition of cell proliferation by DNA synthesis inhibitor cytosine arabinoside completely canceled the IGF-1's cell differentiation promotion, suggesting the possibility that IGF-1's differentiation-promotion effect is an indirect effect via IGF-1's cell proliferation promotion. IGF-1 promoted differentiation dose dependently with maximum effect at 100-500 ng/ml. RT-PCR analysis revealed the upregulation (11-fold) of ifg1 mRNA expression in developing limbs, suggesting that IGF-1 plays a role in promoting muscle differentiation during limb development. The combined effect of triiodo-L-thyronine (T3) and IGF-1 was also examined. In adult leg cells, IGF-1 promoted growth and differentiation irrespective of the presence of T3. In larval tail cells, cell count was 76% lower in the presence of T3, and IGF-1 did not promote proliferation and differentiation in T3-containing medium. In larval dorsal cells, cell count was also lower in the presence of T3, but IGF-1 enhanced proliferation and differentiation in T3-containing medium. This result is likely due to the presence among dorsal cells of both adult and larval types (1:1). Thus, IGF-1 affects only adult-type myogenic cells in the presence of T3 and helps accelerate dorsal muscle remodeling during metamorphosis.
Adams,
The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy.
1996, Pubmed
Adams,
The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy.
1996,
Pubmed
Adams,
Localized infusion of IGF-I results in skeletal muscle hypertrophy in rats.
1998,
Pubmed
Adi,
Early stimulation and late inhibition of extracellular signal-regulated kinase 1/2 phosphorylation by IGF-I: a potential mechanism mediating the switch in IGF-I action on skeletal muscle cell differentiation.
2002,
Pubmed
Allen,
Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor-beta, insulin-like growth factor I, and fibroblast growth factor.
1989,
Pubmed
Brunk,
Assay for nanogram quantities of DNA in cellular homogenates.
1979,
Pubmed
Daughaday,
Somatomedin: proposed designation for sulphation factor.
1972,
Pubmed
Duclos,
Stimulation of DNA synthesis in chicken muscle satellite cells by insulin and insulin-like growth factors: evidence for exclusive mediation by a type-I insulin-like growth factor receptor.
1991,
Pubmed
Díaz,
Expression of rainbow trout glucose transporters GLUT1 and GLUT4 during in vitro muscle cell differentiation and regulation by insulin and IGF-I.
2009,
Pubmed
Engert,
Proliferation precedes differentiation in IGF-I-stimulated myogenesis.
1996,
Pubmed
Gabillard,
In vitro characterization of proliferation and differentiation of trout satellite cells.
2010,
Pubmed
Guo,
MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation.
1995,
Pubmed
Honegger,
Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities.
1986,
Pubmed
Huang,
Timing of metamorphosis and the onset of the negative feedback loop between the thyroid gland and the pituitary is controlled by type II iodothyronine deiodinase in Xenopus laevis.
2001,
Pubmed
,
Xenbase
Jacquemin,
IGF-1 induces human myotube hypertrophy by increasing cell recruitment.
2004,
Pubmed
Lo,
Reversal of muscle differentiation during urodele limb regeneration.
1993,
Pubmed
Machida,
Forkhead transcription factor FoxO1 transduces insulin-like growth factor's signal to p27Kip1 in primary skeletal muscle satellite cells.
2003,
Pubmed
Mathews,
Regulation of insulin-like growth factor I gene expression by growth hormone.
1986,
Pubmed
McFarland,
The influence of growth factors on turkey embryonic myoblasts and satellite cells in vitro.
1993,
Pubmed
Nishikawa,
Isoform transition of contractile proteins related to muscle remodeling with an axial gradient during metamorphosis in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Nishikawa,
Spatial, temporal and hormonal regulation of programmed muscle cell death during metamorphosis of the frog Xenopus laevis.
1995,
Pubmed
,
Xenbase
Perrini,
The GH/IGF1 axis and signaling pathways in the muscle and bone: mechanisms underlying age-related skeletal muscle wasting and osteoporosis.
2010,
Pubmed
Rando,
Primary mouse myoblast purification, characterization, and transplantation for cell-mediated gene therapy.
1994,
Pubmed
Richard-Parpaillon,
The IGF pathway regulates head formation by inhibiting Wnt signaling in Xenopus.
2002,
Pubmed
,
Xenbase
Rinderknecht,
The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.
1978,
Pubmed
Rodgers,
mTORC1 controls the adaptive transition of quiescent stem cells from G0 to G(Alert).
2014,
Pubmed
Rommel,
Mediation of IGF-1-induced skeletal myotube hypertrophy by PI(3)K/Akt/mTOR and PI(3)K/Akt/GSK3 pathways.
2001,
Pubmed
Rosenthal,
Opposing early and late effects of insulin-like growth factor I on differentiation and the cell cycle regulatory retinoblastoma protein in skeletal myoblasts.
1995,
Pubmed
Samuels,
Depletion of L-3,5,3'-triiodothyronine and L-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone.
1979,
Pubmed
Schmid,
Preferential enhancement of myoblast differentiation by insulin-like growth factors (IGF I and IGF II) in primary cultures of chicken embryonic cells.
1983,
Pubmed
Semsarian,
Insulin-like growth factor (IGF-I) induces myotube hypertrophy associated with an increase in anaerobic glycolysis in a clonal skeletal-muscle cell model.
1999,
Pubmed
Shibota,
Larval-to-adult conversion of a myogenic system in the frog, Xenopus laevis, by larval-type myoblast-specific control of cell division, cell differentiation, and programmed cell death by triiodo-L-thyronine.
2000,
Pubmed
,
Xenbase
Shimizu-Nishikawa,
Regulation of specific developmental fates of larval- and adult-type muscles during metamorphosis of the frog Xenopus.
2002,
Pubmed
,
Xenbase
Yamane,
Adult-type myogenesis of the frog Xenopus laevis specifically suppressed by notochord cells but promoted by spinal cord cells in vitro.
2011,
Pubmed
,
Xenbase
Yamane,
Differential muscle regulatory factor gene expression between larval and adult myogenesis in the frog Xenopus laevis: adult myogenic cell-specific myf5 upregulation and its relation to the notochord suppression of adult muscle differentiation.
2013,
Pubmed
,
Xenbase
