Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2016 May 06;29119:9939-47. doi: 10.1074/jbc.M116.723932.
Show Gene links
Show Anatomy links
Regulation of CNGA1 Channel Gating by Interactions with the Membrane.
Aman TK
,
Gordon SE
,
Zagotta WN
.
???displayArticle.abstract???
Cyclic nucleotide-gated (CNG) channels are expressed in rod photoreceptors and open in response to direct binding of cyclic nucleotides. We have previously shown that potentiation of CNGA1 channels by transition metals requires a histidine in the A' helix following the S6 transmembrane segment. Here, we used transition metal ion FRET and patch clamp fluorometry with a fluorescent, noncanonical amino acid (3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap)) to show that the potentiating transition metal Co(2+) binds in or near the A' helix. Adding high-affinity metal-binding sites to the membrane (stearoyl-nitrilotriacetic acid (C18-NTA)) increased potentiation for low Co(2+) concentrations, indicating that the membrane can coordinate metal ions with the A' helix. These results suggest that restraining the A' helix to the plasma membrane potentiates CNGA1 channel opening. Similar interactions between the A' helix and the plasma membrane may underlie regulation of structurally related hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium subfamily H (KCNH) channels by plasma membrane components.
Bradley,
Functional expression of the heteromeric "olfactory" cyclic nucleotide-gated channel in the hippocampus: a potential effector of synaptic plasticity in brain neurons.
1997, Pubmed
Bradley,
Functional expression of the heteromeric "olfactory" cyclic nucleotide-gated channel in the hippocampus: a potential effector of synaptic plasticity in brain neurons.
1997,
Pubmed
Burns,
Activation, deactivation, and adaptation in vertebrate photoreceptor cells.
2001,
Pubmed
Charles,
cAMP modulates the excitability of immortalized H=hypothalamic (GT1) neurons via a cyclic nucleotide gated channel.
2001,
Pubmed
Chatterjee,
A genetically encoded fluorescent probe in mammalian cells.
2013,
Pubmed
Craven,
CNG and HCN channels: two peas, one pod.
2006,
Pubmed
Decher,
Voltage-dependent gating of hyperpolarization-activated, cyclic nucleotide-gated pacemaker channels: molecular coupling between the S4-S5 and C-linkers.
2004,
Pubmed
,
Xenbase
Fesenko,
Induction by cyclic GMP of cationic conductance in plasma membrane of retinal rod outer segment.
,
Pubmed
Flynn,
Molecular mechanism underlying phosphatidylinositol 4,5-bisphosphate-induced inhibition of SpIH channels.
2011,
Pubmed
Ganetzky,
The eag family of K+ channels in Drosophila and mammals.
1999,
Pubmed
Gordon,
Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane.
2016,
Pubmed
Gordon,
A histidine residue associated with the gate of the cyclic nucleotide-activated channels in rod photoreceptors.
1995,
Pubmed
,
Xenbase
Gordon,
Localization of regions affecting an allosteric transition in cyclic nucleotide-activated channels.
1995,
Pubmed
,
Xenbase
Gordon,
Subunit interactions in coordination of Ni2+ in cyclic nucleotide-gated channels.
1995,
Pubmed
,
Xenbase
Horrocks,
Energy transfer between terbium (III) and cobalt (II) in thermolysin: a new class of metal--metal distance probes.
1975,
Pubmed
Hsieh,
Monitoring protein misfolding by site-specific labeling of proteins in vivo.
2014,
Pubmed
Hua,
Functional interactions between A' helices in the C-linker of open CNG channels.
2005,
Pubmed
,
Xenbase
Ildefonse,
Single-channel study of the cGMP-dependent conductance of retinal rods from incorporation of native vesicles into planar lipid bilayers.
1991,
Pubmed
Johnson,
Rotational movement during cyclic nucleotide-gated channel opening.
2001,
Pubmed
,
Xenbase
Kalstrup,
Dynamics of internal pore opening in K(V) channels probed by a fluorescent unnatural amino acid.
2013,
Pubmed
,
Xenbase
Karpen,
Interactions between divalent cations and the gating machinery of cyclic GMP-activated channels in salamander retinal rods.
1993,
Pubmed
Kesters,
Structure of the SthK carboxy-terminal region reveals a gating mechanism for cyclic nucleotide-modulated ion channels.
2015,
Pubmed
Kingston,
Widespread expression of olfactory cyclic nucleotide-gated channel genes in rat brain: implications for neuronal signalling.
1999,
Pubmed
Latt,
Surveyor substrates: energy-transfer gauges of active center topography during catalysis.
1970,
Pubmed
Latt,
Distance measurements at the active site of carboxypeptidase A during catalysis.
1972,
Pubmed
Lee,
Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae.
2009,
Pubmed
Li,
Bimodal regulation of an Elk subfamily K+ channel by phosphatidylinositol 4,5-bisphosphate.
2015,
Pubmed
Michalakis,
Altered synaptic plasticity and behavioral abnormalities in CNGA3-deficient mice.
2011,
Pubmed
Puljung,
A secondary structural transition in the C-helix promotes gating of cyclic nucleotide-regulated ion channels.
2013,
Pubmed
Richmond,
Engineered metal binding sites on green fluorescence protein.
2000,
Pubmed
Sandtner,
In vivo measurement of intramolecular distances using genetically encoded reporters.
2007,
Pubmed
,
Xenbase
Taraska,
Mapping the structure and conformational movements of proteins with transition metal ion FRET.
2009,
Pubmed
Taraska,
Short-distance probes for protein backbone structure based on energy transfer between bimane and transition metal ions.
2009,
Pubmed
Trudeau,
Dynamics of Ca2+-calmodulin-dependent inhibition of rod cyclic nucleotide-gated channels measured by patch-clamp fluorometry.
2004,
Pubmed
,
Xenbase
Wen,
Site-specific fluorescence spectrum detection and characterization of hASIC1a channels upon toxin mambalgin-1 binding in live mammalian cells.
2015,
Pubmed
Womack,
Do phosphatidylinositides modulate vertebrate phototransduction?
2000,
Pubmed
,
Xenbase
Yu,
Accurate high-throughput structure mapping and prediction with transition metal ion FRET.
2013,
Pubmed
Zagotta,
Structural basis for modulation and agonist specificity of HCN pacemaker channels.
2003,
Pubmed
Zagotta,
Gating of single Shaker potassium channels in Drosophila muscle and in Xenopus oocytes injected with Shaker mRNA.
1989,
Pubmed
,
Xenbase
Zagotta,
Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET.
2016,
Pubmed
