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Nat Protoc January 1, 2017; 12 (2): 372-389.

Spinal cord regeneration in Xenopus laevis.

Edwards-Faret G , Muñoz R , Méndez-Olivos EE , Lee-Liu D , Tapia VS , Larraín J .

Here we present a protocol for the husbandry of Xenopus laevis tadpoles and froglets, and procedures to study spinal cord regeneration. This includes methods to induce spinal cord injury (SCI); DNA and morpholino electroporation for genetic studies; in vivo imaging for cell analysis; a swimming test to measure functional recovery; and a convenient model for screening for new compounds that promote neural regeneration. These protocols establish X. laevis as a unique model organism for understanding spinal cord regeneration by comparing regenerative and nonregenerative stages. This protocol can be used to understand the molecular and cellular mechanisms involved in nervous system regeneration, including neural stem and progenitor cell (NSPC) proliferation and neurogenesis, extrinsic and intrinsic mechanisms involved in axon regeneration, glial response and scar formation, and trophic factors. For experienced personnel, husbandry takes 1-2 months; SCI can be achieved in 5-15 min; and swimming recovery takes 20-30 d.

PubMed ID: 28102835
Article link: Nat Protoc

Species referenced: Xenopus laevis
Genes referenced: dpt
Morpholinos: sox2 MO2 sox2 MO5

Article Images: [+] show captions
References [+] :
Beattie, Metamorphosis alters the response to spinal cord transection in Xenopus laevis frogs. 1991, Pubmed, Xenbase