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XB-ART-53013
Dev Biol March 1, 2017; 423 (1): 66-76.

The Nedd4 binding protein 3 is required for anterior neural development in Xenopus laevis.

Kiem LM , Dietmann P , Linnemann A , Schmeisser MJ , Kühl SJ .


Abstract
The Fezzin family member Nedd4-binding protein 3 (N4BP3) is known to regulate axonal and dendritic branching. Here, we show that n4bp3 is expressed in the neural tissue of the early Xenopus laevis embryo including the eye, the brain and neural crest cells. Knockdown of N4bp3 in the Xenopus anterior neural tissue results in severe developmental impairment of the eye, the brain and neural crest derived cranial cartilage structures. Moreover, we demonstrate that N4bp3 depletion leads to a significant reduction of both eye and brain specific marker genes and reduced neural crest cell migration. Finally, we demonstrate an impact of N4bp3 deficiency on cell apoptosis and proliferation. Our studies indicate that N4bp3 is required for early anterior neural development of vertebrates. This is in line with a study implicating that genetic disruption of N4BP3 in humans might be related to neurodevelopmental disease.

PubMed ID: 28104388
Article link: Dev Biol

Genes referenced: adamts2 cpeb4 egr2 emx1l en2 foxd3 hnrnpab n4bp3 nedd4 nhp2 otx2 pax6 pou4f1 ppp2ca prox1 rax rho sar1b sec24a snai2 sox3 stc2 tcf7 twist1 vdac1 vsx1
Morpholinos: n4bp3 MO1 n4bp3 MO2


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