Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The kinase Mps1, long known to be the 'boss' in mitotic checkpoint signaling, phosphorylates multiple proteins in the checkpoint signaling cascade.
Figure 1. The kinase Mps1 and its role in mitotic checkpoint signaling.(A) Mps1 phosphorylates (P) three different proteins to promote the assembly of the mitotic checkpoint complex. It phosphorylates the kinetochore protein KNL1 to recruit the checkpoint protein complex Bub1-Bub3 to KNL1 (1). It phosphorylates Bub1, which allows this protein to interact with another checkpoint protein, Mad1 (2). It also phosphorylates Mad1, which promotes the binding of Mad2 to the regulatory protein Cdc20 (3). Ji et al. propose that phosphorylated Mad1 binds to Cdc20, thereby positioning the latter for capture by Mad2. (B) The checkpoint (represented by the STOP sign) is only active when Mps1 has phosphorylated all three proteins, KNL1, Bub1, and Mad1. (C) Checkpoint activity (y-axis) plotted as a function of Mps1 kinase activity (x-axis) for the phosphorylation of one (P), two (PP) or all three sites (PPP).
Aravamudhan,
The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.
2015, Pubmed
Aravamudhan,
The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.
2015,
Pubmed
Faesen,
Basis of catalytic assembly of the mitotic checkpoint complex.
2017,
Pubmed
Ferrell,
Ultrasensitivity part II: multisite phosphorylation, stoichiometric inhibitors, and positive feedback.
2014,
Pubmed
Hardwick,
Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption.
1996,
Pubmed
Heinrich,
Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
2014,
Pubmed
Hiruma,
CELL DIVISION CYCLE. Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling.
2015,
Pubmed
Ji,
CELL DIVISION CYCLE. Kinetochore attachment sensed by competitive Mps1 and microtubule binding to Ndc80C.
2015,
Pubmed
Ji,
A sequential multi-target Mps1 phosphorylation cascade promotes spindle checkpoint signaling.
2017,
Pubmed
,
Xenbase
Kruse,
A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment.
2014,
Pubmed
London,
Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint.
2014,
Pubmed
Mora-Santos,
Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.
2016,
Pubmed
Musacchio,
The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics.
2015,
Pubmed
Yuan,
Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.
2017,
Pubmed