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XB-ART-53157
Regeneration (Oxf) February 1, 2017; 4 (1): 21-35.

JAK-STAT pathway activation in response to spinal cord injury in regenerative and non-regenerative stages of Xenopus laevis.

Tapia VS , Herrera-Rojas M , Larrain J .


Abstract
Xenopus laevis tadpoles can regenerate the spinal cord after injury but this capability is lost during metamorphosis. Comparative studies between pre-metamorphic and metamorphic Xenopus stages can aid towards understanding the molecular mechanisms of spinal cord regeneration. Analysis of a previous transcriptome-wide study suggests that, in response to injury, the JAK-STAT pathway is differentially activated in regenerative and non-regenerative stages. We characterized the activation of the JAK-STAT pathway and found that regenerative tadpoles have an early and transient activation. In contrast, the non-regenerative stages have a delayed and sustained activation of the pathway. We found that STAT3 is activated in response to injury mainly in Sox2/3(+) ependymal cells, motoneurons and sensory neurons. Finally, to study the role of temporal activation we generated a transgenic line to express a constitutively active version of STAT3. The sustained activation of the JAK-STAT pathway in regenerative tadpoles reduced the expression of pro-neurogenic genes normally upregulated in response to spinal cord injury, suggesting that activation of the JAK-STAT pathway modulates the fate of neural progenitors.

PubMed ID: 28316792
PMC ID: PMC5350081
Article link: Regeneration (Oxf)

Genes referenced: aldh1l1 ccnd2 csf2rb csf3r fos ghr ifngr2.1 ifngr2.2 il10rb il13ra2 il2rg il6st irf9 isl1 jak1 lep lepr lif myc nes neurog2 neurog3 pik3r5 pim1 ptpn6 ptprc socs3 sox2 stam stat1 stat3.1 stat3.2 stat6 vim vim.2

Antibodies referenced: Isl1/2 Ab1 Ptprc Ab1 Sox2 Ab7 phospho-Stat3 Ab2

External Resources:
Article Images: [+] show captions

References:
Anderson, 2016, Pubmed [+]


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