XB-ART-53288Nat Commun. June 23, 2015; 6 7476.
Zic1 controls placode progenitor formation non-cell autonomously by regulating retinoic acid production and transport.
All cranial placode progenitors arise from a common precursor field anterior to the neural plate, the pre-placodal region (PPR). We showed that transcription factor Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. Here we reveal the non-cell autonomous activity of Zic1 and implicate retinoic acid (RA) signalling as a key player in cranial placode progenitor specification. In a screen for genes activated by Zic1, we identify several factors involved in RA metabolism and function. Among them we show that retinaldehyde dehydrogenase 2 (RALDH2) and lipocalin-type prostaglandin D2 synthase (LPGDS), which, respectively, regulate the synthesis and transport of RA, directly participate in the establishment of the PPR. We propose that RALDH2 and LPGDS induction by Zic1 at the anterior neural plate allows for the localized production and transport of RA, which in turn activates a cranial placode developmental programme in neighbouring cells.
PubMed ID: 26101153
Article link: Nat Commun.
Grant support: R01 DE014212 NIDCR NIH HHS, R01-DE014212 NIDCR NIH HHS, R01 DE014212 NIDCR NIH HHS, R01 DE025806 NIDCR NIH HHS, R01-DE014212 NIDCR NIH HHS
Genes referenced: aldh1a2 zic1