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PLoS One January 1, 2014; 9 (3): e92217.

Spatial and temporal control of transgene expression in zebrafish.

Akerberg AA , Stewart S , Stankunas K .

Transgenic zebrafish research has provided valuable insights into gene functions and cell behaviors directing vertebrate development, physiology, and disease models. Most approaches use constitutive transgene expression and therefore do not provide control over the timing or levels of transgene induction. We describe an inducible gene expression system that uses new tissue-specific zebrafish transgenic lines that express the Gal4 transcription factor fused to the estrogen-binding domain of the human estrogen receptor. We show these Gal4-ERT driver lines confer rapid, tissue-specific induction of UAS-controlled transgenes following tamoxifen exposure in both embryos and adult fish. We demonstrate how this technology can be used to define developmental windows of gene function by spatiotemporal-controlled expression of constitutively active Notch1 in embryos. Given the array of existing UAS lines, the modular nature of this system will enable many previously intractable zebrafish experiments.

PubMed ID: 24643048
PMC ID: PMC3958484
Article link: PLoS One
Grant support: [+]
Genes referenced: dnai1 dusp6 krt61 lgals4.2 myc myl7 notch1

Article Images: [+] show captions
References [+] :
Appel, Delta-mediated specification of midline cell fates in zebrafish embryos. 1999, Pubmed

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