Ge X , Grotjahn D , Welch E , Lyman-Gingerich J , Holguin C , Dimitrova E , Abrams EW , Gupta T , Marlow FL , Yabe T , Adler A , Mullins MC , Pelegri F .

5F32GM77835 NIGMS NIH HHS , R01 GM056326 NIGMS NIH HHS , R01GM56326 NIGMS NIH HHS , R01 GM065303 NIGMS NIH HHS , T32-GM007133 NIGMS NIH HHS , T32 GM007133 NIGMS NIH HHS , R01GM065303 NIGMS NIH HHS , T32-HD007516 NICHD NIH HHS , R01HD050901 NICHD NIH HHS , F32 GM077835 NIGMS NIH HHS , T32 HD007516 NICHD NIH HHS , R01 HD050901 NICHD NIH HHS

	Figure 1. Axis induction defects in embryos from mothers homozygous for three different hecate mutant alleles.A–F) Side views (except as indicated in (F)) of live embryos at 24 hpf showing a range of phenotypes from most severe (A) to normal (E) and double-axis embryos (F). A) Class V4 embryo exhibiting complete radial symmetry and lacking all anterior (e.g. head structures) or dorsalmost (e.g. notochord) structures. B) Class V3 embryo exhibiting a rudimentary axis but lacking all anterior and dorsalmost structures. C) Class V2 embryo lacking anteriormost structures such as the eyes, but showing formation of more posterior head structures such as the otic vesicles (black arrowhead, also indicated in D). D) Class V1 embryo with a relatively complete axis but reduced anterior structures, such as the eyes (white arrowhead, also labeled in E), and lacking a properly formed notochord. E) Normal embryo derived from hec mutant females indistinguishable from wild-type. Note somites in (E) are chevron-shaped, while they are blocky in (B–D) indicative of defects in notochord formation, or encircling the entire embryo in (A). F) A double-axis embryo found in hec mutant clutches exhibiting weak expressivity. Insert in the lower left shows the left axis indicated by the dashed rectangle, out of focus in the main image. Insert in the upper left panel shows a dorsal view, showing the bifurcated axis. Note the lack of defined anterior structures in both axes, as well as the lack of a notochord along the trunk, also reflected by block-shaped somites in this region. Images in A–F are side views, except for upper left insert in (F). G–I) Animal views of live wild-type embryos (G) and embryos from females homozygous for each of the three hec mutant alleles. The dorsal thickening or shield (arrow) is absent in mutant embryos. K–V) In situ hybridization analysis to detect expression of dorsally-expressed genes (gsc, chd) and ventrally-expressed genes (eve 1). The expression domains of gsc and cho is reduced in hec mutant embryos, while the expression domain of eve is expanded in these embryos. (K–V) are animal view of embryos, dorsal to the right when identifiable, at the shield stage (6 hpf). Magnification bars in (F) and (V) correspond to 100 µm for panels sets (A–F) and (G–V), respectively. Dorsal view insert in panel (F, upper left) has been reduced to 75% size.
	Figure 2. Molecular identification of the hecate locus.A) Linkage map of the hec locus. The number of recombinants over the total number of analyzed meiosis is indicated. hec linkage was initially identified between SSLP markers z59658 and z24511 on chromosome 8. Fine mapping analysis with newly identified RFLP markers further narrowed the region between the gene gpd1a-1 and the RFLP zC150E8y. B) Contig of five BAC clones covering the hec critical region. CH73-233M11, CH73-272M14, CH73-250D21, DKEY-43H14 and CH211-150E8 are five sequenced and overlapping BAC clones in this interval. C) Exon-intron structure of the hec/grip2a gene, which contains 16 exons. The hecp06ucal, hect2800 and hecp08ajug alleles each cause a premature stop-codon in exon 4, exon 10 and exon 12, respectively. D) Sequence traces of the cDNA products from wild-type and the three mutant hec alleles. Nucleotide substitutions are indicated by the red box. Mutant cDNAs show a C-A transversion in codon 118 (hecp06ucal), a C-T transversion in codon 414 (hect2800), or a C-T transversion in codon 499 (hecp08ajug), all creating premature STOP codons. E) Schematic diagram showing the protein domain structures of Grip2a in the wild-type and mutant alleles. Red boxes represent conserved PDZ domains.
	Figure 3. Whole mount in situ hybridization analysis of the expression of zebrafish grip2a mRNA in early embryos.A–E) grip2a mRNA is localized in a slightly off-center position at the vegetal pole of early embryos, and remains in that position until the sphere stage when it becomes undetectable. F) Control sense probe. G) The domain of grip2a mRNA localization in hec mutants is reduced in intensity and appears to lack an off-center shift. H,I) Representative control (DMSO)- and nocodazole-treated embryos showing the lack of off-center shift in nocodazole-treated embryos (see Figure S4 for details). All panels are side views (except for (B), which is a vegetal view of the embryo in (A)) at the following stages: A,B: 1-cell (20 mpf), C,F,G: 2-cell (45 mpf), D: 64-cell (2 hpf), E: sphere (4 hpf). (H,I) are at 40 mpf, which approximately corresponds to the 2-cell stage (C) in untreated embryos. Magnification bar in (I) corresponds to 100 µm for all panels.
	Figure 4. Localization of grip2a mRNA in wild-type and mutant oocytes.A–C) Whole mount in situ hybridization of dissected ovaries from wild-type (A), bucky ball (buc, B) and magellan (mgn, C) mutant females. In (A–C) oocytes at stage III of development are indicated. Smaller oocytes are at stages I and II, which are difficult to differentiate in whole mounts at this magnification. The grip2a mRNA localization domain is observed in an asymmetric cortical position in wild-type oocytes (A) but is unlocalized and diffuse in buc oocytes (B) and internally-located in mgn mutants (C). D–J) Sections of wild-type and mutant oocytes at the indicated stages after labeling to detect grip2a mRNA. Stages are as indicated in the panel and were determined by size and oocyte morphology according to [101]. D–F) Wild-type oocytes showing localization to the presumptive Balbiani Body (D) and subsequent localization to a cortical domain of the oocyte corresponding to the presumptive vegetal pole (E–F). G,H) buc mutant oocytes lack the Balbiani body [39], [43] and the grip2a mRNA subcellular localization domain in stage I and II oocytes. I) mgn mutant stage I oocytes exhibit an enlarged Balbiani body [40] and displayed an enlarged grip2a mRNA localization domain. J) Stage II mgn mutant oocytes fail to localize transcripts to the vegetal pole which instead persist in an internal domain [40], as observed also for grip2a mRNA. Number of oocytes examined were as follows: wild-type: early stage I: 13, stage II: 12; buc: early stage I: 35, stage II: 26; mgn: early stage I: 23, stage II: 18. Magnification bar in (C) corresponds to 250 µm for panels (A–C), and in (J) to 50 µm for panels (D–J).
	Figure 5. Microtubule reorganization at the vegetal cortex is affected in hecate mutants.A–F) Cortical microtubule network at the vegetal pole in wild-type (A) and hec mutant (B–F) embryos at 20 mpf. Microtubules appear oriented in the same direction and bundled in wild-type embryos (A). The extent of co-orientation and bundling is greatly reduced in hec mutant embryos (B), where microtubules form multiple aster-like structures which can have a well focused-center (C,D) or can exhibit a central microtubule-free zone (E,F) and often overlap (F) or interdigitate (D). The relatively unbundled microtubule arrangement shown in (B) also corresponds to a sector of a large aster-like structure emanating from a not shown central core. Up to 6 aster-like structures were observed in the vegetal cortex of a single embryo. G,H) Cortex in mediolateral regions shows a loose and apparently random network of microtubules which appears similar in both wild-type (G) and hec mutant (H) embryos (n = 8 for wild-type and mutants). All images are z-axis projections of confocal image stages. The phenotype was fully (100%) penetrant according to the two main categories (wild-type, aligned and bundled microtubules; mutant, radialy oriented and unbundled microtubules, with 10 wt and 25 hec mutant embryos imaged. Magnification bar in (H) corresponds to 40 µm for all panels.
	Figure 6. Defects in the vegetal localization of wnt8a mRNA and Sybu protein.A–D) Off-center shift of wnt8a mRNA is affected in hec mutants. Whole mount in situ hybridization of wild-type embryos (A,C) and hec mutant embryos (B,D) at the 1- (A,B, 30 mpf) and 4- (C,D, 60 mpf) cell stages. Images show representative embryos. A majority of wild-type embryos showed a clear off-center shift (85%, n = 27 at 30 mpf and 74%, n = 47 at 60 mpf). A majority of hec mutant embryos showed vegetal localization without a shift at 30 mpf (79%, n = 33, remaining embryos show no localization) and absence of localization at 60 mpf (89%, n = 38, remaining embryos show reduced vegetal localization without a shift). The apparent label at the base of the blastodisc is observed in a majority of mutant embryos (71%, n = 38) but not in wild-type (C) or control embryos labeled with other probes (not shown) and may reflect remaining wnt8a mRNA that has lost anchoring at the vegetal pole and has moved animally through the action of axial streamers [83]. E–I) Localization of Sybu protein is affected in hec mutants. Whole mount immunofluorescence to detect Sybu protein of untreated wild-type (E,G) and hec mutant (F,H,) embryos and nocodazole-treated wild-type embryos (I) at the indicated stages. In wild-type embryos, an off-center shift in Sybu protein can be observed starting at 30 mpf (G). In hec mutants, Sybu protein becomes undetectable levels by this same time point (H). Patterns of localization of Sybu protein at 10 mpf and 20 mpf time points (combined n: 32 WT, 19 mutant for 10–20 mpf), and 30 mpf and 40 mpf time points were similar and have been combined. 59% (n = 32) of wild-type and 63% (n = 19) of hec mutant embryos showed centered vegetal localization during 10–20 mpf. At 30–40 mpf, the percent of embryos that showed vegetal localization, now with an off-center shift, was reduced to 25% (n = 28) in wild-type, and 0% (n = 25) of hec mutants showed any localization at these time points. Treatment of wild-type embryos with nocodazole inhibits the shift but does not result in delocalization from the vegetal cortex (I, embryo at 40 mpf), as previously shown [6]. Magnification bars in (D) and (I) correspond to 100 µm for panels sets (A–D) and (E–I), respectively.
	Figure 7. Long-range animally-directed transport is not affected in hecate mutants.A–C) Paths of injected 0.2 um fluorescent beads after injection into the vegetal pole with a single (A,B) or double (C) injection in wild-type (A,C) or hec mutant (B) embryos. (A′–C′) show merged imaged including the fluorescent channel (shown in A–C) and corresponding DIC optics at low intensity. The extent and frequency of bead transport appeared similar in wild-type and mutant embryos (A,B, see text). Injections into two opposite sides of the vegetal pole results in multiple animally-directed paths, indicating that the entirety of the mediolateral cortex is competent for bead movement. Arrowheads and arrows in (A–C) indicate site of injection in the vegetal region and animally-directed paths along mediolateral regions, respectively. Magnification bar in (C′) corresponds to 100 µm for all panels.
	Figure 8. Germ plasm recruitment and PCG determination appears unaffected in hecate mutants.A–F) Whole mount in situ hybridization of 4-cell stage (60 mpf) embryos to label the germ line-specific genes dazl1 (A,B,D,E), and vasa (C,F) in wild-type (A–C) and hec mutant (D–F) embryos. Early localization of dazl mRNA to the vegetal pole (A,D, side views), and dazl mRNA and vasa mRNA to the germ plasm as it becomes recruited to the furrows of the first and second cleavage cycles (B,C,E,F, animal views). Localization of dazl domains of recruitment at the furrow is not detected in the side views as the levels of mRNA in these domains are relatively low and the focal plane is not optimal for their visualization. G,H) PGC determination, as determined by vasa expression in 24-hour embryos (G), appears unaffected in hec mutants (H), although the PGCs in mutant embryos do not become clustered as in wild-type due to the aberrant cell specification in these mutants. Quantification of the number of vasa-expressing cells is presented in Figure S9. Magnification bar in (H) corresponds to 100 µm for all panels.
	Figure 9. Amplification of hecate/grip2a-dependent symmetry breaking event by a general animal-directed long-range transport system.A) Cortical shifts of various vegetally localized components, including wnt8a mRNA, Sybu protein and grip2a mRNA ([6], [7], [8]; this report) are short-range and dependent on microtubule bundling and alignment, itself dependent on hec function. In wild-type embryos, such a short-range shift generates a symmetry breaking event that is subsequently amplified by long-range, animally-directed transport mechanism independent of hec function and not restricted to the prospective dorsal axis. B) In hec mutant embryos, neither reorganization of vegetal microtubules into aligned bundles nor a short-range shift occur, so that, even though long-range transport remains intact, vegetal determinant transport to the animal pole is affected. The mechanistic basis for the long-range transport, occurring in the region of a loosely organized mediolateral microtubule cortical network remains to be determined (see Discussion).

Ataman, Nuclear trafficking of Drosophila Frizzled-2 during synapse development requires the PDZ protein dGRIP. 2006, Pubmed

Ataman, Nuclear trafficking of Drosophila Frizzled-2 during synapse development requires the PDZ protein dGRIP. 2006, Pubmed
Bard, Glutamate receptor dynamics and protein interaction: lessons from the NMDA receptor. 2011, Pubmed
Bellipanni, Essential and opposing roles of zebrafish beta-catenins in the formation of dorsal axial structures and neurectoderm. 2006, Pubmed
Bontems, Bucky ball organizes germ plasm assembly in zebrafish. 2009, Pubmed
Braat, Characterization of zebrafish primordial germ cells: morphology and early distribution of vasa RNA. 1999, Pubmed
Cai, Syntabulin-kinesin-1 family member 5B-mediated axonal transport contributes to activity-dependent presynaptic assembly. 2007, Pubmed
Cai, Syntabulin-mediated anterograde transport of mitochondria along neuronal processes. 2005, Pubmed
Carney, Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes. 2010, Pubmed
Cha, Wnt5a and Wnt11 interact in a maternal Dkk1-regulated fashion to activate both canonical and non-canonical signaling in Xenopus axis formation. 2008, Pubmed , Xenbase
Cha, Wnt11/5a complex formation caused by tyrosine sulfation increases canonical signaling activity. 2009, Pubmed , Xenbase
Chan, The maternally localized RNA fatvg is required for cortical rotation and germ cell formation. 2007, Pubmed , Xenbase
Chan, fatvg encodes a new localized RNA that uses a 25-nucleotide element (FVLE1) to localize to the vegetal cortex of Xenopus oocytes. 1999, Pubmed , Xenbase
Christoffels, Fugu genome analysis provides evidence for a whole-genome duplication early during the evolution of ray-finned fishes. 2004, Pubmed
Cox, A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis. 2003, Pubmed , Xenbase
Cuykendall, Vegetally localized Xenopus trim36 regulates cortical rotation and dorsal axis formation. 2009, Pubmed , Xenbase
De Brabander, Taxol induces the assembly of free microtubules in living cells and blocks the organizing capacity of the centrosomes and kinetochores. 1981, Pubmed
De Robertis, Dorsal-ventral patterning and neural induction in Xenopus embryos. 2004, Pubmed , Xenbase
Dong, Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2. 1999, Pubmed
Donovan, Cortical granule exocytosis is coupled with membrane retrieval in the egg of Brachydanio. 1986, Pubmed
Dosch, Maternal control of vertebrate development before the midblastula transition: mutants from the zebrafish I. 2004, Pubmed
Elinson, A transient array of parallel microtubules in frog eggs: potential tracks for a cytoplasmic rotation that specifies the dorso-ventral axis. 1988, Pubmed , Xenbase
Eno, Gradual recruitment and selective clearing generate germ plasm aggregates in the zebrafish embryo. 2013, Pubmed
Fagni, Identification and functional roles of metabotropic glutamate receptor-interacting proteins. 2004, Pubmed
Fink, DomainDraw: a macromolecular feature drawing program. 2007, Pubmed
Fuentes, Ooplasmic segregation in the zebrafish zygote and early embryo: pattern of ooplasmic movements and transport pathways. 2010, Pubmed
Furukawa, Structure and function of glutamate receptor amino terminal domains. 2012, Pubmed
Gazave, Posterior elongation in the annelid Platynereis dumerilii involves stem cells molecularly related to primordial germ cells. 2013, Pubmed
Gordon, Wnt signaling: multiple pathways, multiple receptors, and multiple transcription factors. 2006, Pubmed
Gupta, Microtubule actin crosslinking factor 1 regulates the Balbiani body and animal-vegetal polarity of the zebrafish oocyte. 2010, Pubmed , Xenbase
Hashimoto, Localized maternal factors are required for zebrafish germ cell formation. 2004, Pubmed
Houliston, Patterns of microtubule polymerization relating to cortical rotation in Xenopus laevis eggs. 1991, Pubmed , Xenbase
Houston, Cortical rotation and messenger RNA localization in Xenopus axis formation. 2012, Pubmed , Xenbase
Ivarsson, Plasticity of PDZ domains in ligand recognition and signaling. 2012, Pubmed
Jesuthasan, Dynamic microtubules and specification of the zebrafish embryonic axis. 1997, Pubmed
Joly, The ventral and posterior expression of the zebrafish homeobox gene eve1 is perturbed in dorsalized and mutant embryos. 1993, Pubmed , Xenbase
Kaneshiro, The mRNA coding for Xenopus glutamate receptor interacting protein 2 (XGRIP2) is maternally transcribed, transported through the late pathway and localized to the germ plasm. 2007, Pubmed , Xenbase
Kelly, Maternally controlled (beta)-catenin-mediated signaling is required for organizer formation in the zebrafish. 2000, Pubmed
Kimmel, Stages of embryonic development of the zebrafish. 1995, Pubmed , Xenbase
Kirilenko, The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. 2008, Pubmed , Xenbase
Kishimoto, The molecular nature of zebrafish swirl: BMP2 function is essential during early dorsoventral patterning. 1997, Pubmed
Knaut, Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification. 2000, Pubmed
Korkut, Trans-synaptic transmission of vesicular Wnt signals through Evi/Wntless. 2009, Pubmed
Kume, Galphas family G proteins activate IP(3)-Ca(2+) signaling via gbetagamma and transduce ventralizing signals in Xenopus. 2000, Pubmed , Xenbase
Larabell, Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in beta-catenin that are modulated by the Wnt signaling pathway. 1997, Pubmed , Xenbase
Lee, PDZ domains and their binding partners: structure, specificity, and modification. 2010, Pubmed
Lehmann, The maternal gene nanos has a central role in posterior pattern formation of the Drosophila embryo. 1991, Pubmed
Lekven, Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning. 2001, Pubmed
Lu, Identification and mechanism of regulation of the zebrafish dorsal determinant. 2011, Pubmed
Lyman Gingerich, hecate, a zebrafish maternal effect gene, affects dorsal organizer induction and intracellular calcium transient frequency. 2005, Pubmed
Lyman Gingerich, Analysis of axis induction mutant embryos reveals morphogenetic events associated with zebrafish yolk extension formation. 2006, Pubmed
Maegawa, Maternal mRNA localization of zebrafish DAZ-like gene. 1999, Pubmed , Xenbase
Marchler-Bauer, CD-Search: protein domain annotations on the fly. 2004, Pubmed
Marlow, Bucky ball functions in Balbiani body assembly and animal-vegetal polarity in the oocyte and follicle cell layer in zebrafish. 2008, Pubmed
Marrari, Complementary roles for dynein and kinesins in the Xenopus egg cortical rotation. 2004, Pubmed , Xenbase
Marrari, Local inhibition of cortical rotation in Xenopus eggs by an anti-KRP antibody. 2000, Pubmed , Xenbase
Mei, hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish. 2009, Pubmed
Mei, Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification. 2013, Pubmed , Xenbase
Miller, Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation. 1999, Pubmed , Xenbase
Mizuno, Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish. 1999, Pubmed
Mohler, Dominant maternal-effect mutations of Drosophila melanogaster causing the production of double-abdomen embryos. 1986, Pubmed
Nojima, Syntabulin, a motor protein linker, controls dorsal determination. 2010, Pubmed
Nojima, Genetic evidence for involvement of maternally derived Wnt canonical signaling in dorsal determination in zebrafish. 2004, Pubmed
Ober, Signals from the yolk cell induce mesoderm, neuroectoderm, the trunk organizer, and the notochord in zebrafish. 1999, Pubmed
Olsen, A vasa-like gene in zebrafish identifies putative primordial germ cells. 1997, Pubmed
Pelegri, Identification of recessive maternal-effect mutations in the zebrafish using a gynogenesis-based method. 2004, Pubmed
Pelegri, Function of zebrafish beta-catenin and TCF-3 in dorsoventral patterning. 1998, Pubmed
Pelegri, Maternal factors in zebrafish development. 2003, Pubmed
Pelegri, A gynogenesis-based screen for maternal-effect genes in the zebrafish, Danio rerio. 1999, Pubmed
Pelegri, Genetic screens for mutations affecting adult traits and parental-effect genes. 2011, Pubmed
Pelegri, A mutation in the zebrafish maternal-effect gene nebel affects furrow formation and vasa RNA localization. , Pubmed , Xenbase
Pepling, Mouse oocytes within germ cell cysts and primordial follicles contain a Balbiani body. 2007, Pubmed
Petersen, Wnt signaling and the polarity of the primary body axis. 2009, Pubmed
Rebscher, Vasa unveils a common origin of germ cells and of somatic stem cells from the posterior growth zone in the polychaete Platynereis dumerilii. 2007, Pubmed
Rowning, Microtubule-mediated transport of organelles and localization of beta-catenin to the future dorsal side of Xenopus eggs. 1997, Pubmed , Xenbase
Saneyoshi, The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos. 2002, Pubmed , Xenbase
Schroeder, Organization and regulation of cortical microtubules during the first cell cycle of Xenopus eggs. 1992, Pubmed , Xenbase
Schulte-Merker, Expression of zebrafish goosecoid and no tail gene products in wild-type and mutant no tail embryos. 1994, Pubmed , Xenbase
Schulte-Merker, The zebrafish organizer requires chordino. 1997, Pubmed , Xenbase
Selman, Stages of oocyte development in the zebrafish, Brachydanio rerio. 1993, Pubmed
Setou, Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites. 2002, Pubmed
Solnica-Krezel, Microtubule arrays of the zebrafish yolk cell: organization and function during epiboly. 1994, Pubmed
St Johnston, The origin of pattern and polarity in the Drosophila embryo. 1992, Pubmed
Su, Syntabulin is a microtubule-associated protein implicated in syntaxin transport in neurons. 2004, Pubmed
Sugiura, PDZ adaptors: their regulation of epithelial transporters and involvement in human diseases. 2011, Pubmed
Swan, Complex interaction of Drosophila GRIP PDZ domains and Echinoid during muscle morphogenesis. 2006, Pubmed
Swan, A glutamate receptor-interacting protein homolog organizes muscle guidance in Drosophila. 2004, Pubmed
Sémon, Reciprocal gene loss between Tetraodon and zebrafish after whole genome duplication in their ancestor. 2007, Pubmed
Takamiya, A direct functional link between the multi-PDZ domain protein GRIP1 and the Fraser syndrome protein Fras1. 2004, Pubmed
Tao, Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos. 2005, Pubmed , Xenbase
Tarbashevich, XGRIP2.1 is encoded by a vegetally localizing, maternal mRNA and functions in germ cell development and anteroposterior PGC positioning in Xenopus laevis. 2007, Pubmed , Xenbase
Theusch, Separate pathways of RNA recruitment lead to the compartmentalization of the zebrafish germ plasm. 2006, Pubmed
Thisse, Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos. 1993, Pubmed
Tran, Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. 2012, Pubmed
Wagner, Maternal control of development at the midblastula transition and beyond: mutants from the zebrafish II. 2004, Pubmed
Wang, nanos function is essential for development and regeneration of planarian germ cells. 2007, Pubmed
Weaver, GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs. 2003, Pubmed , Xenbase
Westfall, Requirement for intracellular calcium modulation in zebrafish dorsal-ventral patterning. 2003, Pubmed
Westfall, Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/beta-catenin activity. 2003, Pubmed
Wu, Chemokine GPCR signaling inhibits β-catenin during zebrafish axis formation. 2012, Pubmed
Yoon, Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells. 1997, Pubmed