XB-ART-53575
Dev Cell
2017 Mar 27;406:595-607.e4. doi: 10.1016/j.devcel.2017.02.017.
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Foxh1 Occupies cis-Regulatory Modules Prior to Dynamic Transcription Factor Interactions Controlling the Mesendoderm Gene Program.
Charney RM
,
Forouzmand E
,
Cho JS
,
Cheung J
,
Paraiso KD
,
Yasuoka Y
,
Takahashi S
,
Taira M
,
Blitz IL
,
Xie X
,
Cho KW
.
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The interplay between transcription factors and chromatin dictates gene regulatory network activity. Germ layer specification is tightly coupled with zygotic gene activation and, in most metazoans, is dependent upon maternal factors. We explore the dynamic genome-wide interactions of Foxh1, a maternal transcription factor that mediates Nodal/TGF-β signaling, with cis-regulatory modules (CRMs) during mesendodermal specification. Foxh1 marks CRMs during cleavage stages and recruits the co-repressor Tle/Groucho in the early blastula. We highlight a population of CRMs that are continuously occupied by Foxh1 and show that they are marked by H3K4me1, Ep300, and Fox/Sox/Smad motifs, suggesting interplay between these factors in gene regulation. We also propose a molecular "hand-off" between maternal Foxh1 and zygotic Foxa at these CRMs to maintain enhancer activation. Our findings suggest that Foxh1 functions at the top of a hierarchy of interactions by marking developmental genes for activation, beginning with the onset of zygotic gene expression.
???displayArticle.pubmedLink??? 28325473
???displayArticle.pmcLink??? PMC5434453
???displayArticle.link??? Dev Cell
???displayArticle.grants??? [+]
R01 HD073179 NICHD NIH HHS , S10 OD021718 NIH HHS , S10 RR025496 NCRR NIH HHS , S10 OD010794 NIH HHS , P30 CA062203 NCI NIH HHS
Species referenced: Xenopus tropicalis
Genes referenced: ep300 foxh1.2
???displayArticle.antibodies??? Sox7 Ab1
???displayArticle.morpholinos??? foxh1 MO2
???displayArticle.gses??? GSE85273: NCBI
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