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XB-ART-53589
Proc Natl Acad Sci U S A January 1, 2017; 114 (15): E3081-E3090.

Spemann organizer transcriptome induction by early beta-catenin, Wnt, Nodal, and Siamois signals in Xenopus laevis.

Ding Y , Ploper D , Sosa EA , Colozza G , Moriyama Y , Benitez MD , Zhang K , Merkurjev D , De Robertis EM .


Abstract
The earliest event in Xenopus development is the dorsal accumulation of nuclear β-catenin under the influence of cytoplasmic determinants displaced by fertilization. In this study, a genome-wide approach was used to examine transcription of the 43,673 genes annotated in the Xenopus laevis genome under a variety of conditions that inhibit or promote formation of the Spemann organizer signaling center. Loss of function of β-catenin with antisense morpholinos reproducibly reduced the expression of 247 mRNAs at gastrula stage. Interestingly, only 123 β-catenin targets were enriched on the dorsal side and defined an early dorsal β-catenin gene signature. These genes included several previously unrecognized Spemann organizer components. Surprisingly, only 3 of these 123 genes overlapped with the late Wnt signature recently defined by two other groups using inhibition by Dkk1 mRNA or Wnt8 morpholinos, which indicates that the effects of β-catenin/Wnt signaling in early development are exquisitely regulated by stage-dependent mechanisms. We analyzed transcriptome responses to a number of treatments in a total of 46 RNA-seq libraries. These treatments included, in addition to β-catenin depletion, regenerating dorsal and ventral half-embryos, lithium chloride treatment, and the overexpression of Wnt8, Siamois, and Cerberus mRNAs. Only some of the early dorsal β-catenin signature genes were activated at blastula whereas others required the induction of endomesoderm, as indicated by their inhibition by Cerberus overexpression. These comprehensive data provide a rich resource for analyzing how the dorsal and ventral regions of the embryo communicate with each other in a self-organizing vertebrate model embryo.

PubMed ID: 28348214
PMC ID: PMC5393196
Article link: Proc Natl Acad Sci U S A
Grant support: [+]
Genes referenced: adam19 admp agr2 aldh1a2 apobec2 arhgap39 bambi bhlhe22 bmp2 cer1 chrd.1 chrna4 cnrip1 cpe crx ctnnb1 ctrl dact1 dennd2c dkk1 dmbx1 dnase1l3 efnb2 egr1 fam110c fam89a fgf20 foxb1 foxd3 frzb frzb2 fzd8 gata4 gata5 gsc hes1 hhex hlx hs6st1 ids irx1 kazald1 krt12.5 lef1 lhx1 lmo1 lmo4.1 lmo4.2 LOC100170590 lpar6 lrig3 lzts1 myf5 nat8.9 nck2 nkx6-2 nodal nodal2 nodal3.1 nodal3.2 nodal5 nodal5.4 nodal6 nog olig4 osr1 otx2 pcdh9 pdgfra pkdcc.1 pkdcc.2 plekhg5 prrt1 prss27 rab20 rgs1 sebox shisa2 sia1 skor1 slc2a1 slc34a2 sncg sox17b.1 sox17b.2 spns2 srrd st3gal5 szl tbl1x tbxt tmc1 tmem150b wnt1 wnt8a zic1 zic2 zic3 zic4
GO keywords: embryo development [+]
Morpholinos: ctnnb1 MO1

GEO Series:
   GSE93195: Xenbase,  NCBI


Article Images: [+] show captions
References:
Agius, 2000, Pubmed, Xenbase [+]


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