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Elife. January 1, 2017; 6

Molecular mechanism of voltage-dependent potentiation of KCNH potassium channels.

Dai G , Zagotta WN .

EAG-like (ELK) voltage-gated potassium channels are abundantly expressed in the brain. These channels exhibit a behavior called voltage-dependent potentiation (VDP), which appears to be a specialization to dampen the hyperexitability of neurons. VDP manifests as a potentiation of current amplitude, hyperpolarizing shift in voltage sensitivity, and slowing of deactivation in response to a depolarizing prepulse. Here we show that VDP of D. rerio ELK channels involves the structural interaction between the intracellular N-terminal eag domain and C-terminal CNBHD. Combining transition metal ion FRET, patch-clamp fluorometry, and incorporation of a fluorescent noncanonical amino acid, we show that there is a rearrangement in the eag domain-CNBHD interaction with the kinetics, voltage-dependence, and ATP-dependence of VDP. We propose that the activation of ELK channels involves a slow open-state dependent rearrangement of the direct interaction between the eag domain and CNBHD, which stabilizes the opening of the channel.

PubMed ID: 28443815
PMC ID: PMC5440166
Article link: Elife.
Grant support: R01 EY010329 NEI NIH HHS , R01 MH102378 NIMH NIH HHS

Genes referenced: kcnh1

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Aman, 2016, Pubmed, Xenbase [+]

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