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XB-ART-53706
Front Endocrinol (Lausanne) January 1, 2017; 8 99.

Leptin Induces Mitosis and Activates the Canonical Wnt/β-Catenin Signaling Pathway in Neurogenic Regions of Xenopus Tadpole Brain.

Bender MC , Sifuentes CJ , Denver RJ .


Abstract
In addition to its well-known role as an adipostat in adult mammals, leptin has diverse physiological and developmental actions in vertebrates. Leptin has been shown to promote development of hypothalamic circuits and to induce mitosis in different brain areas of mammals. We investigated the ontogeny of leptin mRNA, leptin actions on cell proliferation in the brain, and gene expression in the preoptic area/hypothalamus of tadpoles of Xenopus laevis. The level of leptin mRNA was low in premetamorphic tadpoles, but increased strongly at the beginning of metamorphosis and peaked at metamorphic climax. This increase in leptin mRNA at the onset of metamorphosis correlated with increased cell proliferation in the neurogenic zones of tadpole brain. We found that intracerebroventricular (i.c.v.) injection of recombinant Xenopus leptin (rxLeptin) in premetamorphic tadpoles strongly increased cell proliferation in neurogenic zones throughout the tadpole brain. We conducted gene expression profiling of genes induced at 2 h following i.c.v. injection of rxLeptin. This analysis identified 2,322 genes induced and 1,493 genes repressed by rxLeptin. The most enriched Kyoto Encyclopedia of Genes and Genomes term was the canonical Wnt/β-catenin pathway. Using electroporation-mediated gene transfer into tadpole brain of a reporter vector responsive to the canonical Wnt/β-catenin signaling pathway, we found that i.c.v. rxLeptin injection activated Wnt/β-catenin-dependent transcriptional activity. Our findings show that leptin acts on the premetamorphic tadpole brain to induce cell proliferation, possibly acting via the Wnt/β-catenin signaling pathway.

PubMed ID: 28533765
PMC ID: PMC5421298
Article link: Front Endocrinol (Lausanne)


Species referenced: Xenopus
Genes referenced: aplp2 arrb2 brd2 cirbp ctnnb1 cxcl8 dab2 dhcr7 dhx8 dnajc8 gca gna11 gtf2h2 h3-3a hmgn1 hsp90aa1.1 idh3a ifrd1 irf1 lep lpar4 mad2l1 mbd3 muc19 myh4 nrn1 pcca phyhipl pin1 prss3 psat1 rpl8 set socs3 sox8 srsf3 tef tifa tmem14a ube2d3 wnt1 zmynd8

GEO Series: GSE97243: NCBI

Article Images: [+] show captions
References [+] :
Ahima, Leptin signaling. 2004, Pubmed