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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis.
Ohk J
,
Jung H
.
???displayArticle.abstract??? Blood vessels supply oxygen and nutrients throughout the body, and the formation of the vascular network is under tight developmental control. The efficient in vivo visualization of blood vessels and the reliable quantification of their complexity are key to understanding the biology and disease of the vascular network. Here, we provide a detailed method to visualize blood vessels with a commercially available fluorescent dye, human plasma acetylated low density lipoprotein DiI complex (DiI-AcLDL), and to quantify their complexity in Xenopus tropicalis. Blood vessels can be labeled by a simple injection of DiI-AcLDL into the beating heart of an embryo, and blood vessels in the entire embryo can be imaged in live or fixed embryos. Combined with gene perturbation by the targeted microinjection of nucleic acids and/or the bath application of pharmacological reagents, the roles of a gene or of a signaling pathway on vascular development can be investigated within one week without resorting to sophisticated genetically engineered animals. Because of the well-defined venous system of Xenopus and its stereotypic angiogenesis, the sprouting of pre-existing vessels, vessel complexity can be quantified efficiently after perturbation experiments. This relatively simple protocol should serve as an easily accessible tool in diverse fields of cardiovascular research.
Augustin,
Control of vascular morphogenesis and homeostasis through the angiopoietin-Tie system.
2009, Pubmed
Augustin,
Control of vascular morphogenesis and homeostasis through the angiopoietin-Tie system.
2009,
Pubmed
Bussmann,
Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk.
2010,
Pubmed
Cha,
Evolutionarily repurposed networks reveal the well-known antifungal drug thiabendazole to be a novel vascular disrupting agent.
2012,
Pubmed
,
Xenbase
Elliott,
Transplantation of Xenopus laevis tissues to determine the ability of motor neurons to acquire a novel target.
2013,
Pubmed
,
Xenbase
Grainger,
Xenopus tropicalis as a model organism for genetics and genomics: past, present, and future.
2012,
Pubmed
,
Xenbase
Herbert,
Molecular control of endothelial cell behaviour during blood vessel morphogenesis.
2011,
Pubmed
Lawson,
Arteries and veins: making a difference with zebrafish.
2002,
Pubmed
Levine,
Fluorescent labeling of endothelial cells allows in vivo, continuous characterization of the vascular development of Xenopus laevis.
2003,
Pubmed
,
Xenbase
Li,
High levels of acetylated low-density lipoprotein uptake and low tyrosine kinase with immunoglobulin and epidermal growth factor homology domains-2 (Tie2) promoter activity distinguish sinusoids from other vessel types in murine bone marrow.
2009,
Pubmed
Longair,
Simple Neurite Tracer: open source software for reconstruction, visualization and analysis of neuronal processes.
2011,
Pubmed
Marshak,
Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates axon arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity.
2007,
Pubmed
,
Xenbase
Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Ny,
A transgenic Xenopus laevis reporter model to study lymphangiogenesis.
2013,
Pubmed
,
Xenbase
Showell,
Egg collection and in vitro fertilization of the western clawed frog Xenopus tropicalis.
2009,
Pubmed
,
Xenbase
Weisgraber,
Role of lysine residues of plasma lipoproteins in high affinity binding to cell surface receptors on human fibroblasts.
1978,
Pubmed
Yang,
Calmodulin Mediates Ca2+-Dependent Inhibition of Tie2 Signaling and Acts as a Developmental Brake During Embryonic Angiogenesis.
2016,
Pubmed
,
Xenbase
