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Anal Biochem January 1, 2017; 531 12-15.

Solid-phase synthesis of highly repetitive chromatin assembly templates.

Blacketer MJ , Gannon MK , Young IA , Shogren-Knaak MA .

DNA templates for assembling chromatin model systems typically consist of numerous repeats of nucleosome positioning sequences, making their synthesis challenging. Here we describe a solid-phase strategy for generating such templates using sequential enzymatic ligation of DNA monomers. Using single nucleosome site monomers, we can either generate a twelve-nucleosome site target, or systematically access intermediate-sized templates. Using twelve nucleosome positioning site monomers, longer templates can be generated. Our synthesized templates assemble into well-defined chromatin model systems, demonstrating the utility of our solid-phase approach. Moreover, our strategy should be more widely applicable to generating other DNAs containing highly repetitive DNA sequences.

PubMed ID: 28495586
Article link: Anal Biochem

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