Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-5382
Science 2003 Apr 25;3005619:633-6. doi: 10.1126/science.1081813.
Show Gene links Show Anatomy links

Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos.

Burns KH , Viveiros MM , Ren Y , Wang P , DeMayo FJ , Frail DE , Eppig JJ , Matzuk MM .


???displayArticle.abstract???
Upon fertilization, remodeling of condensed maternal and paternal gamete DNA occurs to form the diploid genome. In Xenopus laevis, nucleoplasmin 2 (NPM2) decondenses sperm DNA in vitro. To study chromatin remodeling in vivo, we isolated mammalian NPM2 orthologs. Mouse NPM2 accumulates in oocyte nuclei and persists in preimplantation embryos. Npm2 knockout females have fertility defects owing to failed preimplantation embryo development. Although sperm DNA decondensation proceeds without NPM2, abnormalities are evident in oocyte and early embryonic nuclei. These defects include an absence of coalesced nucleolar structures and loss of heterochromatin and deacetylated histone H3 that normally circumscribe nucleoli in oocytes and early embryos, respectively. Thus, Npm2 is a maternal effect gene critical for nuclear and nucleolar organization and embryonic development.

???displayArticle.pubmedLink??? 12714744
???displayArticle.link??? Science
???displayArticle.grants??? [+]

Species referenced: Xenopus laevis
Genes referenced: npm1 npm2