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XB-ART-53828
Int J Dev Biol January 1, 2017; 61 (6-7): 415-425.

Apolipoprotein C-I mediates Wnt/Ctnnb1 signaling during neural border formation and is required for neural crest development.

Yokota C , Åstrand C , Takahashi S , Hagey DW , Stenman JM .


Abstract
In vertebrates, the neural crest and placodes originate in the neural border, which is located between the neural plate and epidermal ectoderm. The neural crest and placodes give rise to a vast array of cell types. Formation of neural crest is a multi-step process, in which Wnt signals are used reiteratively, but it is currently not clear if a Wnt signal is required for neural border formation. Here, we have identified apolipoprotein C-I (apoc1) in a screen for genes regulated by Wnt/Ctnnb1 signaling in late blastula stage Xenopus tropicalis embryos. We show that Xenopus laevis apoc1 encodes a small, secreted protein, and is induced by Wnt/Ctnnb1 signaling. Depletion of Apoc1 protein results in a neural border formation defect and loss of border fates, including neural crest cells. However, unlike another Wnt/Ctnnb1 target, gbx2.2, apoc1 is not required for patterning of the neural border. We further show that gbx2.2 and apoc1 are independently regulated by Wnt signaling. Our results thus suggest that Wnt regulates border formation and patterning by distinct genetic mechanisms.

PubMed ID: 28695961
Article link: Int J Dev Biol

Genes referenced: apoc1 axin1 chrd.1 chrd.2 ctnnb1 dkk1 foxd3 foxi4.1 foxi4.2 gbx2.1 gbx2.2 id3 krt12.4 lrp6 msx1 myc nodal3.2 nodal3.4 odc1 pax3 snai1 snai2 sox2 sox9 twist1 wnt1 wnt3a zic1
GO keywords: ectodermal cell fate determination [+]
Morpholinos: apoc1 MO1 apoc1 MO2 gbx2.2 MO1


Article Images: [+] show captions


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