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Nat Struct Mol Biol
2017 Aug 01;248:672-681. doi: 10.1038/nsmb.3434.
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Musashi 1 regulates the timing and extent of meiotic mRNA translational activation by promoting the use of specific CPEs.
Weill L
,
Belloc E
,
Castellazzi CL
,
Méndez R
.
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The translational reactivation of maternal mRNAs encoding meiotic drivers in vertebrates is accomplished mainly by cytoplasmic polyadenylation. The cytoplasmic polyadenylation elements (CPEs) present in the 3' untranslated regions (3' UTRs) of these transcripts, together with their cognate CPE-binding proteins (CPEBs), define a combinatorial code that determines the timing and extent of translational activation upon meiosis resumption. In addition, the RNA-binding protein Musashi1 (Msi1) regulates polyadenylation of CPE-containing mRNAs by a yet undefined CPEB-dependent or CPEB-independent mechanism. Here we show that Msi1 alone does not support cytoplasmic polyadenylation, but its binding triggers the remodeling of RNA structure, thereby exposing adjacent CPEs and stimulating polyadenylation. In this way, Msi1 directs the preferential use of specific CPEs, which in turn affects the timing and extent of polyadenylation during meiotic progression. Genome-wide analysis of CPEB1- and Msi1-associated mRNAs identified 491 common targets, thus revealing a new layer of CPE-mediated translational control.
Afroz,
A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins.
2014, Pubmed
Afroz,
A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins.
2014,
Pubmed
Arumugam,
Autoregulation of Musashi1 mRNA translation during Xenopus oocyte maturation.
2012,
Pubmed
,
Xenbase
Arumugam,
Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression.
2010,
Pubmed
,
Xenbase
Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase
Battelli,
The RNA-binding protein Musashi-1 regulates neural development through the translational repression of p21WAF-1.
2006,
Pubmed
Belloc,
A deadenylation negative feedback mechanism governs meiotic metaphase arrest.
2008,
Pubmed
,
Xenbase
Carvalho,
A framework for oligonucleotide microarray preprocessing.
2010,
Pubmed
Charlesworth,
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes.
2004,
Pubmed
,
Xenbase
Charlesworth,
A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
2002,
Pubmed
,
Xenbase
Charlesworth,
Musashi regulates the temporal order of mRNA translation during Xenopus oocyte maturation.
2006,
Pubmed
,
Xenbase
Coll,
A novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis.
2010,
Pubmed
Curanovic,
Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap.
2013,
Pubmed
Eliscovich,
Spindle-localized CPE-mediated translation controls meiotic chromosome segregation.
2008,
Pubmed
,
Xenbase
Gentleman,
Bioconductor: open software development for computational biology and bioinformatics.
2004,
Pubmed
Gunter,
Translational control in germ cell development: A role for the RNA-binding proteins Musashi-1 and Musashi-2.
2011,
Pubmed
,
Xenbase
Igea,
Meiosis requires a translational positive loop where CPEB1 ensues its replacement by CPEB4.
2010,
Pubmed
,
Xenbase
Imai,
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA.
2001,
Pubmed
Katz,
Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state.
2014,
Pubmed
Kim,
Antagonism between GLD-2 binding partners controls gamete sex.
2009,
Pubmed
Kuwako,
Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
2010,
Pubmed
Lagadec,
The RNA-binding protein Musashi-1 regulates proteasome subunit expression in breast cancer- and glioma-initiating cells.
2014,
Pubmed
Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase
Menon,
Rho GTPase-independent regulation of mitotic progression by the RhoGEF Net1.
2013,
Pubmed
Messias,
Structural basis of single-stranded RNA recognition.
2004,
Pubmed
Novoa,
Mitotic cell-cycle progression is regulated by CPEB1 and CPEB4-dependent translational control.
2010,
Pubmed
,
Xenbase
Okabe,
Translational repression determines a neuronal potential in Drosophila asymmetric cell division.
2001,
Pubmed
Paris,
Maturation-specific polyadenylation and translational control: diversity of cytoplasmic polyadenylation elements, influence of poly(A) tail size, and formation of stable polyadenylation complexes.
1990,
Pubmed
,
Xenbase
Piqué,
A combinatorial code for CPE-mediated translational control.
2008,
Pubmed
,
Xenbase
Richter,
CPEB: a life in translation.
2007,
Pubmed
,
Xenbase
Rutledge,
Efficient translation of Dnmt1 requires cytoplasmic polyadenylation and Musashi binding elements.
2014,
Pubmed
,
Xenbase
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Weill,
Translational control by changes in poly(A) tail length: recycling mRNAs.
2012,
Pubmed
Weill,
A new type of IRES within gag coding region recruits three initiation complexes on HIV-2 genomic RNA.
2010,
Pubmed
Wilkinson,
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE): quantitative RNA structure analysis at single nucleotide resolution.
2006,
Pubmed
Zearfoss,
A conserved three-nucleotide core motif defines Musashi RNA binding specificity.
2014,
Pubmed
Zuker,
Mfold web server for nucleic acid folding and hybridization prediction.
2003,
Pubmed
