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Mol Cell Proteomics
2010 Feb 01;92:336-50. doi: 10.1074/mcp.M900308-MCP200.
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Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition.
Ozlü N
,
Monigatti F
,
Renard BY
,
Field CM
,
Steen H
,
Mitchison TJ
,
Steen JJ
.
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The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that bind selectively to microtubules in C phase and identified several novel binding partners including nucleolar and spindle-associated protein. C phase-selective microtubule binding of many of these proteins depended on activity of Aurora kinases as assayed by treatment with the drug VX680. Aurora-B binding partners switched dramatically between M phase to C phase, and we identified several novel C phase-selective Aurora-B binding partners including PRC1, KIF4, and anaphase-promoting complex/cyclosome. Our approach can be extended to other cellular compartments and cell states, and our data provide the first broad biochemical framework for understanding C phase. Concretely, we report a central role for Aurora-B in regulating the C phase cytoskeleton.
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