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Elife January 1, 2017; 6

Ingression-type cell migration drives vegetal endoderm internalisation in the Xenopus gastrula.

Wen JW , Winklbauer R .

During amphibian gastrulation, presumptive endoderm is internalised as part of vegetal rotation, a large-scale movement that encompasses the whole vegetal half of the embryo. It has been considered a gastrulation process unique to amphibians, but we show that at the cell level, endoderm internalisation exhibits characteristics reminiscent of bottle cell formation and ingression, known mechanisms of germ layer internalisation. During ingression proper, cells leave a single-layered epithelium. In vegetal rotation, the process occurs in a multilayered cell mass; we refer to it as ingression-type cell migration. Endoderm cells move by amoeboid shape changes, but in contrast to other instances of amoeboid migration, trailing edge retraction involves ephrinB1-dependent macropinocytosis and trans-endocytosis. Moreover, although cells are separated by wide gaps, they are connected by filiform protrusions, and their migration depends on C-cadherin and the matrix protein fibronectin. Cells move in the same direction but at different velocities, to rearrange by differential migration.

PubMed ID: 28826499
PMC ID: PMC5589415
Article link: Elife
Grant support: MOP-53075 CIHR

Genes referenced: cfp efnb1 mapre1 rab5a rab5c

Morpholinos referenced: cdh3 MO1 efnb1 MO1

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Article Images: [+] show captions

Amack, 2012, Pubmed, Xenbase [+]

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