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XB-ART-53983
Biol Open. October 15, 2017; 6 (10): 1528-1540.

Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration.

Simon E , Thézé N , Fédou S , Thiébaud P , Faucheux C .


Abstract
Drosophila Vestigial is the founding member of a protein family containing a highly conserved domain, called Tondu, which mediates their interaction with members of the TEAD family of transcription factors (Scalloped in Drosophila). In Drosophila, the Vestigial/Scalloped complex controls wing development by regulating the expression of target genes through binding to MCAT sequences. In vertebrates, there are four Vestigial-like genes, the functions of which are still not well understood. Here, we describe the regulation and function of vestigial-like 3 (vgll3) during Xenopus early development. A combination of signals, including FGF8, Wnt8a, Hoxa2, Hoxb2 and retinoic acid, limits vgll3 expression to hindbrain rhombomere 2. We show that vgll3 regulates trigeminal placode and nerve formation and is required for normal neural crest development by affecting their migration and adhesion properties. At the molecular level, vgll3 is a potent activator of pax3, zic1, Wnt and FGF, which are important for brain patterning and neural crest cell formation. Vgll3 interacts in the embryo with Tead proteins but unexpectedly with Ets1, with which it is able to stimulate a MCAT driven luciferase reporter gene. Our findings highlight a critical function for vgll3 in vertebrate early development.

PubMed ID: 28870996
PMC ID: PMC5665465
Article link: Biol Open.

Genes referenced: ets1 fgf8 hoxa2 hoxb2 mcat pax3 tead1 vgll3 wnt8a zic1

Morpholinos referenced: tead1 MO1 tead1 MO2 vgll3 MO1 vgll3 MO2 vgll3 MO3 vgll3 MO4

References:
Aamar, 2008, Pubmed[+]


Article Images: [+] show captions

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