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XB-ART-53984
Development. January 1, 2017; 144 (20): 3755-3765.

Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex.

Aguero T , Jin Z , Chorghade S , Kalsotra A , King ML , Yang J .


Abstract
In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.

PubMed ID: 28870987
PMC ID: PMC5675448
Article link: Development.
Grant support: R01 GM111816 NIGMS NIH HHS , R01 GM102397 NIGMS NIH HHS , R01 HL126845 NHLBI NIH HHS , R21 HD072340 NICHD NIH HHS

Genes referenced: dnd1 eif3a nanos1


External Resources:
Article Images: [+] show captions

References:
Ahringer, 1991, Pubmed[+]


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