Bim gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development.
Low nephron endowment at birth has been associated with an increased risk for developing hypertension and chronic kidney disease. We demonstrated in an earlier study that conditional deletion of the microRNA (miRNA)-processing enzyme Dicer from nephron progenitors results in premature depletion of the progenitors and increased expression of the proapoptotic protein Bim (also known as Bcl-2L11). In this study, we generated a compound mouse model with conditional deletion of both Dicer and Bim, to determine the biologic significance of increased Bim expression in Dicer-deficient nephron progenitors. The loss of Bim partially restored the number of nephron progenitors and improved nephron formation. The number of progenitors undergoing apoptosis was significantly reduced in kidneys with loss of a single allele, or both alleles, of Bim compared to mutant kidneys. Furthermore, 2 miRNAs expressed in nephron progenitors (miR-17 and miR-106b) regulated Bim levels in vitro and in vivo Together, these data suggest that miRNA-mediated regulation of Bim controls nephron progenitor survival during nephrogenesis, as one potential means of regulating nephron endowment.-Cerqueira, D. M., Bodnar, A. J., Phua, Y. L., Freer, R., Hemker, S. L., Walensky, L. D., Hukriede, N. A., Ho, J. Bim gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development.
PubMed ID: 28446592
PMC ID: PMC5503708
Article link: FASEB J.
Grant support: P30 DK079307 NIDDK NIH HHS , R01 DK069403 NIDDK NIH HHS , R00 DK087922 NIDDK NIH HHS , K99 DK087922 NIDDK NIH HHS , T32 DK061296 NIDDK NIH HHS , R01 DK103776 NIDDK NIH HHS , R01 HD053287 NICHD NIH HHS
Genes referenced: bcl2l11 dicer1 pax2 ret six2